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I2018 Sigma

Monoclonal Anti-Insulin antibody produced in mouse

clone K36AC10, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies for Pancreatic Stem Cells,
species reactivity   horse, canine, rat, human, bovine, monkey, feline, rabbit, guinea pig, sheep, pig
application(s)   dot blot: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human or animal pancreas
  radioimmunoassay: suitable
clone   K36AC10, monoclonal
antibody form   ascites fluid
isotype   IgG1
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... INS(3630)
biological source   mouse
conjugate   unconjugated

Description

General description

The antibody reacts specifically against insulin by RIA and immunocytochemistry. It exhibits cross-reactivity with human proinsulin. The antibody binds to insulin with an affinity constant of 8.8 × 109 M-1 in RIA.

Application

Monoclonal Anti-Insulin antibody produced in mouse was used in ELISA-based protein pin array assay to study the interactions between human αB crystalline and insulin. It was used for flow cytometric analysis of rat white blood cells and mast cells.

Features and Benefits

Antibody Bioguarantee
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Biochem/physiol Actions

Insulin is a major regulatory hormone important in the metabolic processes, transport of glucose, amino acids and certain ions. It is also involved in the synthesis of nucleic acids and proteins. The biological effects of insulin are mediated by insulin receptor and it integrates multiple major signaling pathways.

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