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I2513 Sigma

Monoclonal Anti-Human IgG1 antibody produced in mouse

clone 8c/6-39, ascites fluid

Synonym: Monoclonal Anti-Human IgG1 (Fc specific)

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Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Human IgG Secondary Antibodies and Conjugates, Primary Antibodies,
biological source   mouse
antibody form   ascites fluid
clone   8c/6-39, monoclonal
contains   15 mM sodium azide
application(s)   indirect ELISA: 1:400
isotype   IgG2a
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

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Application

Mouse monoclonal clone clone 8c/6-39 anti-Human IgG1 antibody may be used for the identification of human IgG1 subclass by means of various immunoassays and direct hemagglutination (HA) and hemagglutination inhibition (HAI) assays, enzyme linked immunosorbent assay (ELISA), immunofluorometric assay (IFMA) and detection of cytoplasmic IgG. The antibody may be useful in the analysis of the distribution of IgG subclasses in various diseases.

General description

Human IgG consists of four subclasses (1-4) that can be recognized by antigen differences in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG respectively. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens. For instance, antipolysaccharide responses are mainly of the IgG2 subclass, while protein antigens give rise to IgG1 and IgG3 antibodies. Lipopolysaccahrides stimulate an IgG2 response in PBL′s and an IgG1 response in the spleen. Human IgG1 is the predominant subclass of in vivo and in vitro produced anti-tetanus toxoid antibodies. Only IgG1 and IgG3 are capable of adherence to mononuclear phagocytes. Serum IgG subclass deficiencies have been recorded for different patient groups. For example, IgG2 and IgG4 deficiency is associated with IgA deficiency as found in patients of ataxia telangiextasia. Low IgG2 levels were found in patients with SLE and juvenile diabetes melitus. A disproportionate elevation of IgG1 has also been found in the cerebral spinal fluid of patients with multiple sclerosis. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and may assist in the diagnosis of various disorders.

The antibody is specific for the Fc portion of human IgG1 and is non-reactive with other IgG subclasses. This clone, also described as HP-6091, was found to exhibit a valuable profile of reactivity and specificity for human IgG1 by the IUIS/WHO study.

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Protocols & Articles

Articles

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