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I2519 Sigma

Monoclonal Anti-Interleukin-8 antibody produced in mouse

clone 6217, purified immunoglobulin, lyophilized powder

Synonym: Anti-CXCL8, Anti-IL-8

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Cytokines, Chemokines, and Growth Factors for Stem Cell Biology, Antibodies against Cytokines/Growth Factors/Chemokines for Stem Cell Biology, Antibodies for Cell Biology,
species reactivity   human
application(s)   capture ELISA: suitable
  flow cytometry: suitable
  immunocytochemistry: 25 μg/mL using using calcium ionophore- and PMA-stimulated human peripheral blood mononuclear cells
  neutralization: suitable
  western blot: 1-2 μg/mL
clone   6217, monoclonal
antibody form   purified immunoglobulin
form   lyophilized powder
isotype   IgG1
storage temp.   −20°C
Gene Information   human ... IL8(3576)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

purified, E. coli-derived, recombinant human IL-8.

General description

Interleukin-8 or CXCL8 (IL-8) is a proinflammatory chemokine that belongs to the C-X-C family. Monocytes and macrophages are the primary sources of IL-8 but it is also secreted by most nucleated cells. IL-8 binds to two G protein-coupled receptors, CXCR1 and CXCR2 and leads to the downstream activation of PI3K, MAPK, PKC, STAT3 and FAK pathways. IL-8 is responsible for the recruitment monocytes and neutrophils that results in a prolonged acute inflammation. The expression of IL-8 is influenced by inflammatory signals, chemical and environmental stress and hormones. Deregulation of IL-8 signaling has been observed in atherosclerosis, cardiovascular disease and solid tumors of bladder, prostate, ovary, glial cells and pancreas. Monoclonal Anti-Interleukin-8 antibody recognizes human IL-8. By immunoblotting, the antibody shows 100% cross reactivity with recombinant porcine IL-8, and no cross reactivity with recombinant rat CINC-2α.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Application

Monoclonal Anti-Interleukin-8 antibody may be used for neutralization assays; ND50 is 0.08-0.4 μg/ml. The antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. For ELISA a working concentration of 0.5 μg/ml may be used. Antibody concentration of 25 μg/ml may be used for immunocytochemistry using stimulated human peripheral blood mononuclear cells. For flow cytometry, 10 μL of 25 μg/ml stock antibody solution may be used for 1-5 X 105 cells.

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