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I2643 Sigma

Insulin human

recombinant, expressed in yeast (proprietary host)

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

25, 50, 250 mg in poly bottle

Application

Insulin human has been used:
• in the culture medium needed for the adipogenesis of ASCs (adipose-derived stem cells)
• to study the effect of insulin in the young and aged brains of rats.
• to study the effect of insulin on the inflammatory actions of protease-activated receptor 2 (PAR2) using mice model.

Biochem/physiol Actions

Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.

Price and Availability


Laboratory Gloves

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
RTECS 
NM8900250

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What solvents can be used to make a solution of Insulin human, Product I2643?
Insulin is readily soluble in dilute acid and alkalies.  The solubility of insulin is tested in 0.01 M hydrochloric acid at a concentration of 20 mg/mL.
How can I store the solutions of Insulin human, Product I2643, and how long?
A stock solution can be stored frozen at -20°C in single-use aliquots. Freeze-thaw cycles should be avoided.  Alternatively, it can be stored for up to 12 months at 2-8°C if it is sterile filtered through a low protein binding membrane or if it contains a suitable bacteriostat, such as 0.1% thimerosal or sodium azide.
How many units of insulin are present in my vial of Insulin human, Product I2643?
The potency is listed by lot on the Certificate of Analysis, which can be accessed through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/search/AdvancedSearchPage?TabSelection=RelatedInformation Generally, product I2643 has a potency of 27.5 units/mg.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

Plasma Derived Proteins and Enzymes

Despite their complexity, blood and plasma are abundant biological resources for the discovery of drug targets and biomarkers for human disease. It is estimated that plasma may contain as many as 40,...
BioFiles 2006, 1.5, 2.
Keywords: Applications, Gas chromatography, Type

Peer-Reviewed Papers
15

References

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