• USA Home
  • I3019 - Interleukin-10 from mouse

EMAIL THIS PAGE TO A FRIEND
I3019 Sigma

Interleukin-10 from mouse

>97% (SDS-PAGE), recombinant, expressed in E. coli, cell culture tested

Synonym: IL-10

Purchase

Properties

Related Categories Cancer Research, Cell Biology, Cell Signaling and Neuroscience, Cytokines & Growth Factors, Cytokines and Growth Factors,
recombinant   expressed in E. coli
assay   >97% (SDS-PAGE)
form   lyophilized powder
mol wt   mol wt ~18 kDa
impurities   endotoxin, tested
suitability   cell culture tested
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Il10(16153)

Description

Analysis Note

The biological activity of IL-10 is determined in a cell proliferation assay using MC/9 cells.

Physical form

lyophilized from 25.0 μL of a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein

Application

Interleukin-10 from mouse has been used as a standard in ELISA (enzyme-linked immunosorbent assay) cytokine assay.

Biochem/physiol Actions

Interleukin-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1+ (CD5+), and normal B cells. In human, the cellular sources of IL-10 consist of CD4+ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.

Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Interleukin Family (IL)

The Interleukins comprise a disparate group of cytokines and growth factors that are produced by and released from leukocytes. Interleukin-1β (1L-1β) is released primarily from stimulated macrophages...
Jennifer Fries
BioFiles 2009, 4.5, 16.
Keywords: Anti-inflammatory agents, Antitumor agents, Cancer, Cell proliferation, Gene expression, Growth factors, Transcription

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?