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I3146 Sigma

ITS Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

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Properties

Related Categories Cell Culture, Hybridoma Media Supplements, Hybridoma Platform, ITS and Other Supplements, Reagents and Supplements,
Quality Level   GMP
sterility   sterile-filtered
product line   BioReagent
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Components

Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.

Other Notes

ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Related Content

Selenium in Cell Culture

Importance and uses of Selenium in serum-free eucaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Keywords: Apoptosis, Catalysis, Cell culture, Culture media, Free radical reactions, Gastrointestinal, Lipid peroxidation, Oxidations, Peroxidations, Reductions

Transferrin in Cell Culture

Importance and uses of transferrins in serum-free eucaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Keywords: Cell culture, Culture media, Gene expression, Glycosylations, Oxidations

Peer-Reviewed Papers
15

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