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  • I3391 - Anti-Human IgG (Fc specific), F(ab′)2 fragment antibody produced in goat

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I3391 Sigma

Anti-Human IgG (Fc specific), F(ab′)2 fragment antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Human IgG Secondary Antibodies and Conjugates, Primary Antibodies,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
concentration   2.0 mg/mL protein
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Other Notes

Antibody adsorbed with mouse and rat IgG

Immunogen

Purified human IgG

Preparation Note

Adsorbed to reduce background with mouse or rat samples.
Useful when trying to avoid background staining due to the presence of Fc receptors.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% sodium azide

Application

Anti-human IgG (Fc specific), F(ab’)2 fragment antibody can be used in ELISA to determine human IgG1-Fc protein.

General description

IgG antibody plays a crucial role in humoral immune responses such as phagocytosis, complement activation, placental transport and cell surface-receptor binding. Anti-human IgG (Fc specific), F(ab’)2 fragment antibody can be used as a capture antibody in antibody-binding assay for soluble ICAM-1-Fc. Goat anti-human-IgG (Fc specific) F(ab’)2 antibody reacts specifically with human IgG but does not react with other immunoglobulins.

Price and Availability


Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

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