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I3642 Sigma

Monoclonal Anti-Interleukin-1β antibody produced in mouse

clone 8516.311, purified immunoglobulin, lyophilized powder

Synonym: Anti-IL-1β

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Description

Immunogen

purifed, E. coli-derived, recombinant human IL-1β.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Specificity

The antibody will neutralize the biological activity of recombinant human IL-1β. It will not neutralize the activity of recombinant human IL-α, recombinant mouse IL-1α, or recombinant mouse IL-1β. When immobilized on a microplate, this antibody will capture both recombinant as well as natural human IL-1β. In immunoblotting, the antibody shows ~25% cross-reactivity with recombinant rat IL-1β and <5% cross-reactivity with recombinant mouse IL-1β and recombinant porcine IL-1β. Additionally, in immunoblotting, the antibody exhibits no cross-reactivity with recombinant mouse IL-α, recombinant human IL-1ra, recombinant mouse IL-1ra, or recombinant rat IL-1α.

Preparation Note

Monoclonal Anti-Interleukin-1(IL-1) (mouse IgG1 isotype) is purified from a mouse hybridoma utilizing Protein A affinity chromatography.

Application

May used in immunoblotting, immunohistochemistry, ELISA, and neutralization assays

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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