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I4639 Sigma

IgG4, Kappa from human myeloma plasma

purified immunoglobulin, >95% (SDS-PAGE)

Synonym: Human IgG4-κ

Properties

Related Categories Antibodies, Human IgG Subclasses, Purified Immunoglobulins, Sera and Immunoglobulins, Supplementary Products More...
antibody form   purified immunoglobulin
assay   >95% (SDS-PAGE)
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Analysis Note

The purity and identity are determined by immunoelectrophoresis, indirect ELISA, and SDS-PAGE.

Physical form

Solution in 0.02 M Tris buffered saline, pH 8.0.

Application

The purified IgG4, κ, may be used as an immunoglobulin calibrator, reference antigen, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot blot immunobinding, Western immunobloting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Purified IgG4, κ, was used as standard in ELISA1,2 and for labelling human IgG subclasses by flow cytometry.3

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.4 IgG4, κ is obtained from human IgG4 κ myeloma sera and purified by fractionation, ion-exchange, and affinity chromatography.

Price and Availability


2015 Sigma-Aldrich Antibody Calendar

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