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I4753 Sigma

Invertase from Candida utilis

Grade X, ≥300 units/mg solid

Synonym: β-D-Fructofuranoside fructohydrolase, β-Fructofuranosidase, Saccharase

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
type   Grade X
mol wt   mol wt ~260 kDa
foreign activity   α-galactosidase (melibiase), essentially free
storage temp.   −20°C

Description

Application

This enzyme is useful for enzymatic determination of saccharose and for the structure investigation of carbohydrates containing β-D-fructofuranoside residue.

Packaging

1 g in poly bottle

Preparation Note

Chromatographically purified

Unit Definition

One unit will hydrolyze 1.0 μmole of sucrose to invert sugar per min at pH 4.5 at 55°C.

Physical properties

Michaelis constant : 1.5x10 -2M (Saccharose)
Structure : Glycoprotein containing ca. 50% of carbohydrates
Optimum pH : 3.5 - 4.0 Optimum temperature : 60 - 70oC
pH Stability : pH 4.0 - 6.0 (50oC, 10min)
Thermal stability : below 60oC (pH 4.5, 10min)
Substrate specificity : The enzyme hydrolyzes saccharose and raffinose, but does not
hydrolyze inulin and melezitose.6)

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Protocols

Assay Procedure for Invertase

The appearance of reducing sugars is measured by the modified Fehling-Lehmann-Schoorl method.7)
Keywords: Indicators, Titrations

Enzymatic Assay of Invertase

This procedure applies to all products that have specification for the enzymatic activity of Invertase at Sigma-Aldrich Saint Louis.
Keywords: Biochemistry, Enzyme activity, Enzymology

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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