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I5020 Sigma

Anti-Interleukin 10 antibody produced in goat

IgG fraction of antiserum, lyophilized powder

Synonym: Anti-IL-10

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors, Antibodies to Interleukins (IL),
species reactivity   human
application(s)   neutralization: suitable
clone   polyclonal
antibody form   IgG fraction of antiserum
form   lyophilized powder
storage temp.   −20°C
Gene Information   human ... IL10(3586)
biological source   goat
conjugate   unconjugated

Description

Immunogen

recombinant human IL-10 expressed in Sf 21 insect cells.

General description

Interleukin-10 (IL-10) is a pleotropic cytokine with anti-inflammatory and immunosuppressive functions. It is the key cytokine that regulates the host inflammatory responses to bacterial, fungal and viral pathogens. The production of IL-10 is mediated by signalling of IL-6, IL-12 and TGF-β. IL-10 is secreted by a variety of cells including T cells, B cells, dendritic cells, eosinophils, natural killer cells and mast cells. The effects of IL-10 are mediated by binding to the IL-10R, consisting of α and β subunits. The signalling through IL-10R activates Jak1, Tyk2 protein kinases and JAK/STAT3 pathways. IL-10 is involved in suppression of innate immunity to kill pathogens and inhibits the release of reactive oxygen and nitrogen species from activated macrophages. Regulation of IL-10 production is important in infections, inflammatory responses in autoimmune encephalomyelitis, endotoxic shock and chronic enterocolitis
Anti-interleukin-10 antibody is specific for human IL-10. The antibody shows ~7% cross reactivity with recombinant mouse IL-10 in ELISA and immunoblotting.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Specificity

The antibody neutralizes the biological activity of recombinant human IL-10, but not recombinant mouse IL-10.

Preparation Note

Purified using protein G.

Application

Anti-interleukin-10 antibody may be used for immunoblotting at a working concentration of 1 μg/ml. For ELISA a working concentration of 0.25 μg/ml is recommended. The antibody is suitable for neutralization reactions. This antibody has also been used for immunohistochemistry of cervicovaginal epithelial cells of rabbit

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