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I5020 Sigma

Anti-Interleukin 10 antibody produced in goat

IgG fraction of antiserum, lyophilized powder

Synonym: Anti-IL-10

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors, Antibodies to Interleukins (IL),
species reactivity   human
application(s)   neutralization: suitable
clone   polyclonal
antibody form   IgG fraction of antiserum
form   lyophilized powder
storage temp.   −20°C
Gene Information   human ... IL10(3586)
biological source   goat
conjugate   unconjugated

Description

Immunogen

recombinant human IL-10 expressed in Sf 21 insect cells.

General description

Interleukin-10 (IL-10) is a pleotropic cytokine with anti-inflammatory and immunosuppressive functions. It is the key cytokine that regulates the host inflammatory responses to bacterial, fungal and viral pathogens. The production of IL-10 is mediated by signalling of IL-6, IL-12 and TGF-β. IL-10 is secreted by a variety of cells including T cells, B cells, dendritic cells, eosinophils, natural killer cells and mast cells. The effects of IL-10 are mediated by binding to the IL-10R, consisting of α and β subunits. The signalling through IL-10R activates Jak1, Tyk2 protein kinases and JAK/STAT3 pathways. IL-10 is involved in suppression of innate immunity to kill pathogens and inhibits the release of reactive oxygen and nitrogen species from activated macrophages. Regulation of IL-10 production is important in infections, inflammatory responses in autoimmune encephalomyelitis, endotoxic shock and chronic enterocolitis
Anti-interleukin-10 antibody is specific for human IL-10. The antibody shows ~7% cross reactivity with recombinant mouse IL-10 in ELISA and immunoblotting.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Specificity

The antibody neutralizes the biological activity of recombinant human IL-10, but not recombinant mouse IL-10.

Preparation Note

Purified using protein G.

Application

Anti-interleukin-10 antibody may be used for immunoblotting at a working concentration of 1 μg/ml. For ELISA a working concentration of 0.25 μg/ml is recommended. The antibody is suitable for neutralization reactions. This antibody has also been used for immunohistochemistry of cervicovaginal epithelial cells of rabbit

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Peer-Reviewed Papers
15

References

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