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I5029 Sigma

IgG1, Lambda from human myeloma plasma

purified immunoglobulin, >95% (SDS-PAGE)

Synonym: Human IgG1-λ

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Properties

Related Categories Antibodies, Human IgG Subclasses, Purified Immunoglobulins, Sera and Immunoglobulins, Supplementary Products More...
biological source   human
antibody form   purified immunoglobulin
assay   >95% (SDS-PAGE)
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Analysis Note

Identity is determined by immunoelectrophoresis and indirect ELISA.

Physical form

Solution in 0.02 M Tris buffered saline, pH 8.0.

Application

IgG1 λ from human myeloma can be used as reference antigen, blocking agent, immunoglobulin calibrator or coating protein in various immunoassay like ELISA, dot blot immunobinding, immunodiffusion, immunoelectrophoresis, hemagglutination, cell-binding assays and western immunoblotting.

The purified IgG1, λ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Human myeloma IgG1, λ was used as non-specific control in quantitative ELISA and solid-phase ELISA. A range of concentrations of IgG1, λ from 10 to 100 ng was used for quantitative ELISA. Human myeloma IgG1, λ was also used as control in size-exclusion chromatography to analyse purity of recombinant antibodies.

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG1 is the most abundant of the IgG subclass
Human myeloma IgG1, λ is purified from human plasma by fractionation, ion-exchange, and affinity chromatography procedures.

IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane. IgG is further subdivided into four subclass- IgG1, IgG2, IgG3 and IgG4.Elevation in antigen specific IgG1 is associated with symptomatic infection and Th2-regulation. IgG1 λ from human myeloma can be used in quantitative ELISA.Human IgG1 λ react specifically with human IgG.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

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