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I5131 Sigma

IgG from sheep serum

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

Synonym: Sheep IgG

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Properties

Related Categories Antibodies, Normal Human and Animal Immunoglobulins, Purified Immunoglobulins, Sera and Immunoglobulins, Supplementary Products More...
biological source   sheep
antibody form   purified immunoglobulin
grade   reagent grade
assay   ≥95% (SDS-PAGE)
form   essentially salt-free, lyophilized powder
isotype   IgG
solubility   saline: soluble1 mg/mL at -20 °C (Repeated freezing and thawing is not recommended.)
conjugate   unconjugated
storage temp.   2-8°C

Description

Analysis Note

Protein concentration should be determined after reconstitution by absorbance at 280 nm (E1%/280 = 14).

Packaging

10, 50, 100 mg in poly bottle

Application

Purified IgG may be used as a reference antigen, standard, blocking agent or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, immunoprecipitation and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid-phase immunoadsorbents.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

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