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I5145 Sigma

Anti-Interleukin-10 antibody produced in goat

IgG fraction of antiserum, lyophilized powder

Synonym: Anti-IL-10

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors, Antibodies to Interleukins (IL),
species reactivity   mouse
application(s)   neutralization: suitable
  western blot: suitable
clone   polyclonal
antibody form   IgG fraction of antiserum
form   lyophilized powder
storage temp.   −20°C
Gene Information   mouse ... Il10(16153)
biological source   goat
conjugate   unconjugated

Description

Immunogen

recombinant mouse IL-10 expressed in E. coli.

General description

Interleukin-10 (IL-10) is a pleotropic cytokine with anti-inflammatory and immunosuppressive functions. It is the key cytokine that regulates the host inflammatory responses to bacterial, fungal and viral pathogens. The production of IL-10 is mediated by signalling of IL-6, IL-12 and TGF-β. IL-10 is secreted by a variety of cells including T cells, B cells, dendritic cells, eosinophils, natural killer cells and mast cells. The effects of IL-10 are mediated by binding to the IL-10R, consisting of α and β subunits. The signalling through IL-10R activates Jak1, Tyk2 protein kinases and JAK/STAT3 pathways. IL-10 is involved in suppression of innate immunity to kill pathogens and inhibits the release of reactive oxygen and nitrogen species from activated macrophages. Regulation of IL-10 production is important in infections, inflammatory responses in autoimmune encephalomyelitis, endotoxic shock and chronic enterocolitis
Anti-interleukin-10 antibody is specific for mouse IL-10. The antibody shows less than 5% cross reactivity to recombinant human IL-10.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Specificity

The antibody neutralizes the biological activity of recombinant mouse IL-10.

Preparation Note

Purified using protein G.

Application

Anti-interleukin-10 antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. The antibody is suitable for neutralization reactions (ND50 is 2-4 μg/ml).

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Safety & Documentation

Documents

Certificate of Analysis

Certificate of Origin

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Peer-Reviewed Papers
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References

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