I5256 Sigma

IgG from goat serum

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

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Synonym: Goat IgG

Properties

Related Categories Antibodies, Normal Human and Animal Immunoglobulins, Purified Immunoglobulins, Sera and Immunoglobulins, Supplementary Products More...
grade   reagent grade
assay   ≥95% (SDS-PAGE)
form   essentially salt-free, lyophilized powder
storage temp.   2-8°C

Description

Application

Purified IgG may be used as a reference antigen, standard, blocking agent or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid-phase immunoadsorbents. Goat IgG was used as negative control in immunohistochemical analysis of human heart tissue samples1 and in preparation of egg-white avidin samples for atomic force microscopy.2 Goat IgG was used as isotype control antibody in immunohistochemical analysis of tissue sections of human tonsils, colon, small intestine, ileus and spleen.3

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections4
Goat IgG is purified from normal goat serum by fractionation and ion-exchange chromatography.

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reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

reagent grade, ≥95% (SDS-PAGE), lyophilized powder

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