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I5395 Sigma

Anti-Interleukin-12 antibody produced in goat

IgG fraction of antiserum, lyophilized powder

Synonym: Anti-IL-12

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Description

Immunogen

recombinant human IL-12 expressed in Sf 21 insect cells.

General description

Interleukin-12 (IL-12) is an important cytokine that links innate and adaptive immunity. It plays a role in the regulation of Th1-associated cellular immunity to foreign pathogens, and humoral immunity to antigens that are both T cell-independent and T cell-dependent. IL-12 is primarily produced by innate immune cells, dendritic cells, epithelial cells and macrophages. All forms of IL-12 bind to the IL-12R which includes IL-12RbI and IL-12RbII subunits expressed on T cells and natural cells. IL-12 stimulates the production of IFN-γ and TNF-α, subsequent production of nitric oxide that leads to pathogen destruction. Reports suggest the involvement of JAK/STAT pathway components in mediating these effects. The effects of IL-12 have been implicated in bacterial, fungal and viral infections. Due to its role in inducing matrix metalloproteases, it also has important role in the etiology of chronic B-cell malignancies and lung adenocarcinomas
Anti-Interleukin-12 antibody recognizes human IL-12.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline

Specificity

The antibody neutralizes the biological activity of recombinant human IL-12.

Preparation Note

Purified using protein G.

Application

Anti-Interleukin-12 antibody may be used for ELISA at a working antibody concentration of 0.5 μg/ml. For immunoblotting, a working concentration of 1 μg/ml is recommended. The antibody is also suitable for neutralization reactions.

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Safety & Documentation

Documents

Certificate of Analysis

Certificate of Origin

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Peer-Reviewed Papers
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References

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