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I5404 Sigma

IgG2, Kappa from human myeloma plasma

purified immunoglobulin, >95% (SDS-PAGE)

Synonym: Human IgG2-κ

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Properties

Related Categories Antibodies, Human IgG Subclasses, Purified Immunoglobulins, Sera and Immunoglobulins, Supplementary Products More...
biological source   human
antibody form   purified immunoglobulin
assay   >95% (SDS-PAGE)
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Analysis Note

Identity is determined by immunoelectrophoresis and indirect ELISA.

Physical form

Solution in 0.02 M Tris buffered saline, pH 8.0.

Application

The purified IgG2, κ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Human myeloma IgG2, κa was used to characterize the trisulfide modifications in human and mouse antibodies and in flow cytometry.

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.
Human myeloma IgG2, κ is purified from human plasma by fractionation, ion-exchange and affinity chromatography procedures.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

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Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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