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  • I5777 - Monoclonal Anti-Interleukin-2 Soluble Receptor β antibody produced in mouse

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I5777 Sigma

Monoclonal Anti-Interleukin-2 Soluble Receptor β antibody produced in mouse

clone 27302.1, purified immunoglobulin, lyophilized powder

Synonym: Anti-IL-2 sRβ

Description

Immunogen

recombinant human IL-2 sRβ, expressed in Sf 21 insect cells.

General description

Interleukin-2 (IL-2) is a cytokine produced by activated T cells in response to antigen stimulation. IL-2 is important for proliferation of lymphocytes, natural killer cells and macrophages and in the differentiation of CD4+ cells into Th1 and Th2 effector cells. The effects of IL-2 are mediated by the signaling activated by its binding to IL-2 receptor (IL-2 R). IL-2 R, which contains three membrane subunits: α, β and γ. The IL-2 R γ is also shared by IL-2, IL-4 and IL-7. The β and the γ subunits complex results in the binding of IL-2 with higher affinity. IL-2 R β is expressed on monocytes, B cells, T cells, NK cells and neutrophils. IL-2 R activates key signaling pathways such as MAPK, JAK/STAT as well as PI3K/Akt. Activation of these pathways modulates production of inflammatory cytokines, survival and progression through cell cycle. Anti-Human IL-2 soluble receptor β (IL-2 sRβ) specifically reacts with IL-2 R β. It does not cross-react with recombinant human IL-2 Rα, IL-2 Rγ, IL-4 sR, and IL-6 sR.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

Specificity

The antibody will neutralize human cell surface IL-2 receptor mediated-bioactivity.

Preparation Note

Purified using protein A.

Application

Anti-Human IL-2 soluble receptor (IL-2 sRγ) antibody may be used in immunoblotting at a working concentration of 1-2 μg/ml. For indirect ELISA, the recommended concentration is 0.5-1.0 μg/ml. The antibody is suitable for IL-2 sRγ neutralization assays.

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