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I6029 Sigma

Interpreting Protein Mass Spectra: A Comprehensive Resource

  •  ISBN-10 0-8412-3571-6

  •  ISBN-13 978-0-8412-3571-7

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publication info   A.P. Snyder, American Chemical Society/ OUP, 2000, 544 pp., hard cover
mfr. no.   American Chemical Society

Description

General description

This guide for researchers investigating structures of proteins and peptides focusses on interpretation of structural information gathered through electrospray ionization-mass spectrometry (ESI-MS). It will also provide background and protocols for anyone using matrix-assisted laser desorption/ionization (MALDI), fast atom bombardment (FAB), and secondary ion mass spectrometry (SIMS). It includes practical examples, discussions of experimental setups, and hints for troubleshooting both methods and handling of materials.

Table of Contents

1. Electrospray Ionization as a Vehicle for Protein Mass Spectra
2. Analyte Separation Methods: High-Pressure Liquid Chromatography and Capillary Electrophoresis
3. The Isotope Effect at High Mass
4. Calculation of the Analyte Mass
5. Analytical Figures of Merit for a High-Mass Isotope Envelope
6. Isotope Distribution of a Multiply Charged Mass Spectral Peak
7. Experimental Parameters Affecting Electrospray Ionization Mass Spectra
8. The Cation Effect on Electrospray Ionization Mass Spectra
9. Interpretation of Peptide and Protein Mass Spectra
10. Interpretation of Phosphoprotein Mass Spectra
11. Immunological Applications: Proteins and Phosphoproteins
12. Interpretation of Glycoprotein Mass Spectra
Appendix 1
Appendix 2
Appendix 3
Glossary
Index

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