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Ion Channel Localization: Methods and Protocols

  •  ISBN-10 0-89603-833-5

  •  ISBN-13 978-0-89603-833-2

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Related Categories Books and Software, Cell Signaling Books, Ion Channel Books, Labware
publication info   A. Lopatin and C.G. Nichols, ed., Humana Press, 2001, 496 pp., hard cover
mfr. no.   Humana

Description

General description

Leading specialists present a comprehensive review of methods in ion channel localization, and bring together in a single book different aspects of the versatile potential of today′s exciting technology. First part of this volume provides a detailed description of the use of Green Fluorescent Protein (GFP). Second part covers issues related to GFP applications for channel localization. Third section is devoted to latest developments in functional mapping of ion channels. Final section provides a comprehensive description of Atomic Force Microscopy.

Table of Contents

Part I. Pharmacological Labeling of Ion Channels and Receptors.
Fluorescent Calcium Antagonists: Tools for Imaging of L-Type Calcium Channels in Living Cells,
Targeting Cerebral Muscarinic Acetylcholine Receptors with Radioligands for Diagnostic Nuclear Medicine Studies,
GABAA Receptors: Autoradiographic Localization of Different Ligand Sites and Identification of Subtypes of the Benzodiazepine Binding Site, .
Localization of ATP P2X Receptors, .
Small Conductance Calcium-Activated Potassium Channels in Rat Brain: Autoradiographic Localization Using Two Specific Toxins, Apamin and Scyllatoxin,
Mapping N-Type Calcium Channel Distributions with w-Conotoxins,
Fluorescent Imaging of Nicotinic Receptors During Neuromuscular Junction Development,
High Affinity Scorpion Toxins for Studying Potassium and Sodium Channels,
Part II. Applications of Green Fluorescent Protein (GFP) Technology.
Bicistronic GFP Expression Vectors as a Tool to Study Ion Channels in Transiently Transfected Cultured Cells,
Confocal Imaging of GFP-Labeled Ion Channels,
Localization and Quantification of GFP-Tagged Ion Channels Expressed in Xenopus Oocytes,
Applications of Green Fluorescent Protein (GFP) Technology: Watching Ion Channel Biogenesis in Living Cells Using GFP Fusion Constructs,
Tagging Ion Channels with the Green Fluorescent Protein (GFP) as a Method for Studying Ion Channel Function in Transgenic Mouse Models,
Mutant GFP-Based FRET Analysis of K+ Channel Organization,
Delivering Ion Channels to Mammalian Cells by Membrane Fusion,
Part III. Functional Assays in Ion Channel Localization.
Detection of Neurons Expressing Calcium-Permeable AMPA Receptors Using Kainate-Induced Cobalt Uptake,
Crystallization Technique for Localizing Ion Channels in Living Cells,.
Imaging of Localized Neuronal Calcium Influx, .
Using Caged Compounds to Map Functional Neurotransmitter Receptors,
Part IV. Atomic Force Microscopy.
Atomic Force Microscopy of Reconstituted Ion Channels,
Imaging the Spatial Organization of Calcium Channels in Nerve Terminals Using Atomic Force Microscopy,
Nanoarchitecture of Plasma Membrane Visualized with Atomic Force Microscopy,
Dynamic AFM of Patch Clamped Membranes,
Localizing Ion Channels with Scanning Probe Microscopes: A Perspective,
Index.

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