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  • I6527 - Anti-Interleukin-4 Soluble Receptor antibody produced in goat

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I6527 Sigma

Anti-Interleukin-4 Soluble Receptor antibody produced in goat

IgG fraction of antiserum, lyophilized powder

Synonym: Anti-IL-4 sR

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors, Antibodies to Interleukins (IL),
species reactivity   human
application(s)   indirect ELISA: suitable
  neutralization: suitable
  western blot: suitable
clone   polyclonal
antibody form   IgG fraction of antiserum
form   lyophilized powder
storage temp.   −20°C
Gene Information   human ... IL4R(3566)
biological source   goat
conjugate   unconjugated

Description

Immunogen

recombinant human IL-4 soluble receptor (IL-4 sR), expressed in Sf 21 insect cells.

General description

Interleukin-4 (IL-4) is a T cell derived chemokine that stimulates the Th2 mediated immune response and proliferation of B cells. The effects of IL-4 are mediated by two types of receptors, type I receptor consisting of IL-4Rα and common γ chain and type II receptor composed of IL-4Rα and IL-13Rα1. The signalling stimulated by IL-4R leads to activation of JAK/STAT6 and IRS-mediated PI3K/Akt pathway. Through these pathways, IL-4 is responsible for endocytic activity of macrophages, chemotaxis of leukocytes in response to inflammation, angiogenesis and regulation of nitric oxide metabolism in macrophages. Anti-tumor effects of IL-4R signaling have been reported in cancers of breast, liver and renal cells
Anti-Interleukin-4 soluble receptor specifically recognizes human cell surface IL-4R.

Physical form

Lyophilized from 51.5 μL of a 0.2μm filitered solution in PBS (pH7.4) with 5% trehalose.

Specificity

The antibody will neutralize human cell surface IL-4 receptor mediated-bioactivity.

Preparation Note

Purified using Protein G.

Application

Anti-Interleukin-4 soluble receptor antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. For ELISA, a working concentration of 0.5-1.0 μg/ml is recommended. The antibody is suitable for neutralization reactions at a working concentration of 5-10 μg/ml. This antibody was used for immunostaining of parasympathetic neurons from guinea pig and human neuroblastoma cells at a dilution of 1:200.

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Safety & Documentation

Documents

Certificate of Analysis

Certificate of Origin

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Peer-Reviewed Papers
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