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I6634 Sigma

Insulin from bovine pancreas

powder, BioReagent, suitable for cell culture

Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Core Bioreagents, Cytokines, Growth Factors and Hormones,
product line   BioReagent
form   powder
potency   ≥27 USP units per mg
suitability   suitable for cell culture
storage temp.   −20°C
Gene Information   cow ... INS(280829)

Description

Frequently Asked Questions

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Application

Insulin is used as a growth factor in many mammalian cell culture systems.

Biochem/physiol Actions

Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.

Reconstitution

Insulin has low solubility at neutral pH. It can be solubilized at 2 mg/ml in dilute acetic or hydrochloric acid, pH 2-3.

Physical form

powder-0 °C; solution stable 2-8 °C, 1 year

Price and Availability

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Technical Service:

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