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  • I7276 - Anti-Insulin-Like Growth Factor-II antibody produced in goat

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I7276 Sigma

Anti-Insulin-Like Growth Factor-II antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-IGF-II

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Cytokines and Growth Factors,
species reactivity   human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5-15 μg/mL using human Placenta
  neutralization: 0.2-0.8 μg/mL
  western blot: suitable
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   −20°C
Gene Information   human ... IGF2(3481)
biological source   goat
conjugate   unconjugated

Description

Immunogen

recombinant human IGF-II

General description

Insulin-like growth factors -I and -II are important regulators of signalling that mediates growth and metabolism in cells. Insulin-like growth factor -II (IGF-II) is widely expressed during embryonic stages and regulates the placental development. IGF-II acts via specific receptor, IGF-IIR, the binding does not result in signal transduction, but is a mechanism to restrict the bioavailability of IGF-II. IGF-II also binds to IGF-IR which results in the activation of MAPK, Raf/Ras and PI3K cascades. The effects of IGF-II signalling are important in growth of blood vessels (angiogenesis) gene transcription, proliferation and transformation. Irregular IGF-II signaling has been implicated in many cancers including lung cancer, colo-rectal cancer, cervical cancer, testicular cancer, rhabdosarcoma, melanoma and hepatoblastoma
Anti-IGF-II recognizes human insulin-like growth factor II. It does not react with human IGF-I.

Physical form

Lyophilized from a 0.2 μm filtered solution of phosphate buffered saline, pH 7.4, with 5% trehalose

Specificity

The antibody will neutralize the biological activity of recombinant human IGF-II. It does not neutralize the biological activity of recombinant human IGF-I.

Application

Anti- IGF-II antibody may be used for neutralization reactions, the ND50 of the antibody is 0.2-0.8 μg/ml. This antibody was used to neutralize IGF-II in glioblastoma cell lines. For immunohistochemistry a working antibody concentration of 5-15 μg/ml may be used for formalin-fixed, paraffin-embedded human placenta sections. The antibody is suitable for immunoblotting.

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