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I7392 Sigma

Monoclonal Anti-Interleukin-10 antibody produced in mouse

clone 23738.11, purified immunoglobulin, lyophilized powder

Synonym: Anti-IL-10

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors, Antibodies to Interleukins (IL),
species reactivity   human
application(s)   neutralization: 2 μg/mL
  western blot: 1-2 μg/mL
clone   23738.11, monoclonal
antibody form   purified immunoglobulin
form   lyophilized powder
isotype   IgG2b
storage temp.   −20°C
Gene Information   human ... IL10(3586)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant human IL-10.

General description

Interleukin-10 (IL-10) is a pleotropic cytokine with anti-inflammatory and immunosuppressive functions. It is the key cytokine that regulates the host inflammatory responses to bacterial, fungal and viral pathogens. The production of IL-10 is mediated by signaling of IL-6, IL-12 and TGF-β. IL-10 is secreted by a variety of cells including T cells, B cells, dendritic cells, eosinophils, natural killer cells and mast cells. The effects of IL-10 are mediated by binding to the IL-10R, consisting of α and β subunits. The signaling through IL-10R activates Jak1, Tyk2 protein kinases and JAK/STAT3 pathways. IL-10 is involved in suppression of innate immunity to kill pathogens and inhibits the release of reactive oxygen and nitrogen species from activated macrophages. Regulation of IL-10 production is important in infections, inflammatory responses in autoimmune encephalomyelitis, endotoxic shock and chronic enterocolitis. Anti-interleukin-10 antibody is specific for human IL-10.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline, pH 7.4, containing 5% trehalose.

Specificity

This antibody will neutralize the biological activity of recombinant human IL-10.

Preparation Note

purified using Protein A or G

Application

Anti-interleukin-10 antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. The antibody is suitable for neutralization assays at a minimum antibody concentration of 2 μg/ml.

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