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  • I7409 - Monoclonal Anti-Interleukin-1α propeptide antibody produced in mouse

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I7409 Sigma

Monoclonal Anti-Interleukin-1α propeptide antibody produced in mouse

clone 409405, purified immunoglobulin, lyophilized powder

Synonym: Anti-IL1, Anti-IL1A

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors, Antibodies to Interleukins (IL),
species reactivity   human
application(s)   western blot: 1-2 μg/mL
clone   409405, monoclonal
antibody form   purified immunoglobulin
form   lyophilized powder
isotype   IgG2b
storage temp.   −20°C
Gene Information   human ... IL1A(3552)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

purified, E. coli-derived recombinant human interleukin-1α propeptide (aa 1-112).

General description

Interleukins are proinflammatory cytokines produced on cell injury or trauma. There are two closely related types of interleukins produced by the cell, IL-1α and IL-1β that are produced by the activation of NF-κ B transcription factor in response to bacterial infection or LPS. Both IL-1α and IL-1β exert their cellular effects by signalling through IL-1 receptor type 1. The IL-1α precursor produced initially is cleaved by calpain to produce a 16 kDa propeptide and 17 kDa mature protein. A variety of cells such as macrophages, epithelial cells, fibroblasts, keratinocytes, monocytes and dendritic cells secrete IL-1α. Both IL-1α and IL-1β are important in acquired immunity, inflammation, response to infection and production of matrix metalloproteinases (MMPs). The propeptide has nuclear localization sequence that enables it to shuttle to nucleus and activate transcription of target genes. The effects of IL-1α are mediated by activation of NF-κB, JNK and MAPK pathways that activated the genes in response to infection and inflammation.Overproduction of IL-1α has been implicated in inflammatory disorders like rheumatoid arthritis and periodontitis
Monoclonal Anti-Interleukin-1a propeptide recognizes human Interleukin-1α propeptide. It does not cross-react with mature forms of mouse IL-1α or rabbit IL-1α.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Application

Anti-interleukin 1α antibody is suitable for immunoblotting at a working concentration of 1-2 μg/ml.

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR  NONH for all modes of transport
WGK Germany  1
Protocols & Articles

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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