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I7526 Sigma

Anti-Interleukin-4 antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-IL-4

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors, Antibodies to Interleukins (IL),
species reactivity   human
application(s)   indirect ELISA: suitable
  neutralization: suitable
  western blot: suitable
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   −20°C
Gene Information   human ... IL4(3565)
biological source   goat
conjugate   unconjugated

Description

Immunogen

recombinant human IL-4.

General description

Interleukin-4 (IL-4) is a T cell derived chemokine that stimulates the Th2 mediated immune response and proliferation of B cells. The effects of IL-4 are mediated by two types of receptors, type I receptor consisting of IL-4Rα and common γ chain and type II receptor composed of IL-4Rα and IL-13Rα1. The signaling stimulated by IL-4 leads to activation of JAK/STAT6 and IRS-mediated PI3K/Akt pathway. Through these pathways, IL-4 is responsible for endocytic activity of macrophages, chemotaxis of leukocytes in response to inflammation, angiogenesis and regulation of nitric oxide metabolism in macrophages. Anti-tumor effects of IL-4 have been reported in cancers of breast, liver and renal cells. Anti-Interleukin-4 specifically recognizes human interleukin-4. The antibody shows no cross reactivity with rmIL-4 and rrIL-4.

Physical form

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Specificity

The antibody will neutralize the biological activity of recombinant human IL-4, but not recombinant mouse IL-4.

Application

Anti-Interleukin-4 antibody may be used for immunoblotting at a working concentration of 0.1-0.2 μg/ml. For ELISA, a working concentration of 0.5-1.0 μg/ml is recommended. The antibody may be used for immunohistochemistry of cultured cells or tissue sections at a working antibody concentration of 0.5-5 μg/ml. The antibody is suitable for neutralization assay; ND50 is 0.02-0.1 μg/ml. Anti-IL-4 antibody was used for immunohistochemistry and ELISA using cervicovaginal tissue sections and cervicovaginal extracts of Belgium white rabbits.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR  NONH for all modes of transport
WGK Germany  2
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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