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  • I7653 - Anti-DFF45, C-Terminal antibody produced in rabbit

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I7653 Sigma

Anti-DFF45, C-Terminal antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-ICAD

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to DNA Fragmentation,
species reactivity   mouse
application(s)   western blot: 1 μg/mL using mouse lung tissue lysate
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 45 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Dffa(13347)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 312-331 of mouse DFF45/ICAD (DNA Fragmentation Factor 45/ Inhibitor of Caspase-3 Activated DNase I).

General description

The gene DFF-45, also referred to as DFFA (DNA fragmentation factor subunit α), encodes the α subunit of the heterodimeric protein DFF (DNA fragmentation factor) that also consists of the β subunit encoded by DFFB. The molar mass of the protein encoded by DFFA is 45kDa. The gene is mapped to human chromosome 1p36.3-p36.2.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide

Biochem/physiol Actions

The protein encoded by the gene DFFA (DNA fragmentation factor subunit α), also referred to as ICAD (inhibitor of caspase-activated deoxyribonuclease), serves as a substrate for caspase-3. It is cleaved at two sites by caspase-3 in response to apoptotic signals, and DFF is activated when DFFA separates from DFFB, the active nuclease that is also referred to as CAD (caspase-activated deoxyribonuclease). DFFA functions as a chaperone facilitating the correct folding of DFFB, and also as an inhibitor of DFFB. Active DFFB functions in the fragmentation of DNA by introducing double-stranded breaks during apoptosis.

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