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  • I7778 - Anti-IKKα (699-715) antibody produced in rabbit

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I7778 Sigma

Anti-IKKα (699-715) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-IκB Kinase α

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, I Kappa B kinase (IKK),
species reactivity   human
application(s)   western blot: 1 μg/mL using human HeLa cell extract
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 85 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... CHUK(1147)
mouse ... Chuk(12675)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 699-715 of human IκB Kinase α (IKK α) (C2). This sequence contains one amino acid substitution with the mouse sequence.

General description

The NF-κB transcription factors are presented inactive in the cytoplasm by inhibitor protein IκB. In response to stimuli such as UV light or inflammatory cytokines, phosphorylation and subsequent degradation of IκB, allows for nuclear translocation of NF-κ B where it functions as transcription factor. IκB is phosphorylated by a serine kinase, IκB kinase (IKK). The IKK consists of three subunits, IKKα and IKKβ are the catalytic, IKKγ is a regulatory subunit. IKKα and its upstream kinase, NIK are the key kinases in the activation of the alternate or the non-canonical NFκB pathway. IKKα has been reported to function independent of either IKKβ or IKKγ in activation of NF-κB. IKKα also shows functions other than activation of NF-κ B. It binds the promoters of estrogen-responsive genes, Cyclin D1 and c-myc
Anti-IKKα?detects IKKα?(85 kDa) and has no cross-reactivity with IKKβ?or IKKγ.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Application

Anti-IKK α subunit may be used for detection by immunoblotting at a recommended concentration of 1 μg/mL or 1:500 dilution using HeLa whole cell lysates.

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