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Immunoassays: A Practical Approach

  •  ISBN-10 0-19-963710-5

  •  ISBN-13 978-0-19-963710-2

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publication info   J. Gosling, ed., Oxford University Press, 2000, 304 pp., soft cover
mfr. no.   Oxford University Press

Description

General description

This is designed to help biologists develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are described. Solid-phase reagents, standards and immunogens are explained, with procedures for their preparation. Most components can now be obtained commercially.

Table of Contents

Protocol List
Abbreviations
1. Analysis by specific binding,
2. Raising antibodies,
3. Choosing and characterizing antibodies,
4. Labels and endpoints,
5. Solid-phase reagents,
6. Standards and immunogens,
7. Optimization,
8. Validation,
9. Data processing,
10. Quality assurance,
A1. Suitable reagents and other requisites
A2. List of suppliers

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