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Imaging Neurons: A Laboratory Manual

  •  ISBN-10 0-87969-541-2

  •  ISBN-13 978-0-87969-541-5

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publication info   R. Yuste, et al., ed., Cold Spring Harbor Laboratory Press, 2000, 838 pp., soft cover
mfr. no.   Cold Spring Harbor

Description

General description

Advances in microscopy, along with new methods of culturing and labeling cells, generated the science of imaging. Imaging technologies allow investigators to look directly inside living cells and probe their form and function in unprecedented detail. This is the first comprehensive description of the range of imaging technologies being applied to living cells. It covers methods and techniques such as multi-photon excitation microscopy and imaging of genetically engineered probes.

Table of Contents

SECTION 1 Microscopes, Image Acquisition, and the Basics of Fluorescence Imaging
Microscopy and Microscope Optical Systems
Arc Lamps and Monochromators for Fluorescence Microscopy
The Use of Liquid-crystal Tunable Filters for Fluorescence Imaging
Acousto-optic Tunable Filters for Microscopy
Video Microscopy, Video Cameras, and Image Enhancement Reprint
The Application of Scientific-grade CCD Cameras to Biological Imaging
Infrared Videomicroscopy
Grating Image Systems for Optical Sectioning Fluorescence Microscopy of Cells, Tissues, and Small Organisms
Analysis of Dynamic Optical Imaging Data
Maintaining Live Cells and Tissue Slices in the Imaging Setup
SECTION 2 Confocal Microscopy
Confocal Microscopy: Principles and Practice
Practical Limits to Resolution in Fluorescence Light Microscopy
In Vivo Imaging of CNS Neuron Development
The Zebrafish Hindbrain: A Transparent System for Imaging Motor Circuitry
Fast Confocal Fluorescence Imaging of Subcellular Calcium Dynamics
Imaging Calcium Sparks in Excitable Cells
SECTION 3 Multiphoton Microscopy
Principles of Multiphoton-excitation Fluorescence Microscopy
Lasers for Multiphoton Microscopy
Two-photon Cross Sections of Indicators
Two-photon Microscopy for 4D Imaging of Living Neurons
Multiphoton Imaging of Embryonic Development
Two-photon Imaging of Neuronal Function in the Neocortex In Vivo
Two-photon Imaging of Neocortical Microcirculation
SECTION 4 Photoactivation
Basics of Photoactivation
Design and Application of Caged Neurotransmitters
Uncaging Calcium in Neurons
Photostimulation with Caged Glutamate
Infrared-guided Photostimulation of Neurons in Brain Slices
Spatially Resolved Flash Photolysis Via Chemical Two-photon Uncaging
SECTION 5 Calcium Imaging
How Calcium Indicators Work
Some Quantitative Aspects of Calcium Fluorimetry
Calibration of Fluorescent Calcium Indicators
A Single-compartment Model of Calcium Dynamics in Nerve Terminals and Dendrites
Loading Brain Slices with AM Esters of Calcium Indicators
Dye Loading with Patch Pipettes
Measuring Calcium Fluxes in Dendritic Domains of Neurons in Brain Slices
Monitoring Presynaptic Calcium Dynamics with Membrane-permeant Indicators
Imaging Intracellular Calcium-concentration Microdomains at a Chemical Synapse
Monitoring Intramitochondrial Calcium with Rhod-2
Generation of Controlled Calcium Oscillations in Nonexcitable Cells
Calcium Imaging of Retinal Activity
Calcium Imaging of Identified Astrocytes in Hippocampal Slices
High-speed Calcium Imaging of Neurons in Brain Slices
In Vivo Calcium Imaging in Blowfly CNS
SECTION 6 Imaging Other Aspects of Neuronal Function
Intrinsic Signal Imaging in the Neocortex
Intrinsic Signal Optical Imaging as a Tool to Visualize the Development ......
Intrinsic Signal Optical Imaging in Brain Slices
Rapid Changes in Light Scattering Associated.......
Imaging with Voltage-sensitive Dyes: .......
Voltage-sensitive-dye Imaging in the Neocortex: ......
Imaging Membrane-potential Changes .......
Imaging Sodium Dynamics in Neurons
Imaging Synaptic Vesicle Dynamics .......
Imaging Exocytosis with Total Internal Reflection Microscopy
SECTION 7 Genetically Engineered Fluorescent Probes
Physiological Indicators Based .....
Cellular Imaging of Bioluminescence
Green Fluorescent Proteins for Measuring Signal Transduction:......
Introduction of Green Fluorescent Protein ......
Imaging Exocytosis with .......
Targeted Recombinant Aequorins..

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