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I8265 Sigma

IgG from sheep serum

technical grade, ≥80% (SDS-PAGE), buffered aqueous solution

Synonym: Sheep IgG

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Properties

Related Categories Antibodies, Normal Human and Animal Immunoglobulins, Purified Immunoglobulins, Sera and Immunoglobulins, Supplementary Products More...
grade   technical grade
assay   ≥80% (SDS-PAGE)
form   buffered aqueous solution
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Packaging

10, 50 mg in glass bottle

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.2, containing 15 mM sodium azide

Application

Technical grade sheep IgG may be used as an economical alternative to the reagent grade immunoglobulins, in applications where high purity is not required. Sheep IgG was used as negative control in 293T cells (human embryonic kidney cells) and Madin-Darby canine kidney (MDCK) cells at a working antibody dilution of 1:40.

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Purified Sheep IgG is isolated from pooled normal sheep serum by fractionation.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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