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I8638 Sigma

Monoclonal Anti-Integrin β1 antibody produced in mouse

clone W1B10, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies for Embryonic Stem Cells,
species reactivity   chicken
application(s)   immunoprecipitation: suitable
  indirect immunofluorescence: suitable
  western blot: 1:200 using denatured, non-reduced extract of cultured fibroblasts from chicken embryo lower leg
clone   W1B10, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 100-140 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   chicken ... ITGB1(374058)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

chicken integrin.1

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin with 15 mM sodium azide.

Specificity

The antibody recognizes an epitope on the extracellular side of the avian integrin complex, and inhibits adhesion of embryo and tendon fibroblasts as well as skeletal myoblasts to both laminin and fibronectin. Under reducing and denaturing conditions using extracts of chicken embryo muscle cells, the antibody precipitates the β1 class integrin heterodimer as a broad band of ~140 kDa. In the absence of reducing agents, the integrin β1 subunit appears as bands at 100 kDa (immature form), 100 kDa (mature form), and 140 kDa (noncovalently associated α-subunits). The antibody may also be used for bioactivity studies in adhesion assays.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
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