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I8640 Sigma

IgG from human serum

technical grade, ≥80% (SDS-PAGE), buffered aqueous solution

Synonym: Human IgG

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Properties

Related Categories Antibodies, Normal Human and Animal Immunoglobulins, Purified Immunoglobulins, Sera and Immunoglobulins, Supplementary Products More...
grade   technical grade
assay   ≥80% (SDS-PAGE)
form   buffered aqueous solution
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.2, containing 15 mM sodium azide

Application

Purified human IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Technical grade human IgG may be used as an economical alternative to the reagent grade immunoglobulins, in applications where high purity is not required. IgG from human serum was used as ELISA standard in various studies. A concentration range of 200 μg/ml to 1 mg/ml of human IgG was used for blocking in flow cytometry.

General description

Human IgG antibodies are primarily responsible for the facilitation of humoral immune responses such as complement fixation, placental transport, and phagocytosis among many others. IgG from human serum can be used as an economical alternative for reagent grade immunoglobulins where high purity is not essential. It can also be used in immunoelectrophoresis. The product is reactive in humans.

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Human IgG is purified from normal human serum by fractionation.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
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06693 Timestrip Plus -20 °C

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