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  • I8773 - Anti-Insulin-Like Growth Factor-I antibody produced in goat

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I8773 Sigma

Anti-Insulin-Like Growth Factor-I antibody produced in goat

affinity isolated antibody

Synonym: Anti-IGF-I

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Cytokines and Growth Factors,
species reactivity   human
application(s)   neutralization: 3-12 μg/mL
  western blot: 0.1-0.2 μg/mL
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   −20°C
Gene Information   human ... IGF1(3479)
biological source   goat
conjugate   unconjugated

Description

Immunogen

recombinant human IGF-I expressed in Escherichia coli

General description

Insulin-like growth factors -1 and -2 are important regulators of signalling that mediates growth and metabolism in cells. Insulin-like growth factor -1 shares structural homology with proinsulin and is mainly produced by liver. IGF-1 acts via specific receptor, IGF-1R, a heterodimeric tyrosine kinase receptor that signals to various second messenger pathways such as MAPK, Raf/Ras and PI3K cascades. The effect of IGF-1 is by endocrine, paracrine and autocrine signalling. The effects of IGF-1 signalling are important at many developmental levels from cells to organ to organism, growth, differentiation, anti-apoptosis, proliferation and transformation. Irregular IGF-1 signaling has been implicated in many cancers including melanoma, breast, lung, colon and prostate
Anti-IGF-I recognizes human insulin-like growth factor I. The antibody does not react with human IGF-II.

Physical form

Lyophilized from a 0.2 μm filtered solution of phosphate buffered saline, pH 7.4, with 5% trehalose.

Specificity

The antibody neutralizes the biological activity of recombinant human IGF-I.

Preparation Note

Affinity isolated by IGF-I affinity chromatography.

Application

Anti- IGF-1 antibody may be used for neutralization reactions, the ND50 of the antibody is 3-12 μg/ml. For immunoblotting a working antibody concentration of 0.1-0.2 μg/ml.

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