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  • I8773 - Anti-Insulin-Like Growth Factor-I antibody produced in goat

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I8773 Sigma

Anti-Insulin-Like Growth Factor-I antibody produced in goat

affinity isolated antibody

Synonym: Anti-IGF-I

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Cytokines and Growth Factors,
species reactivity   human
application(s)   neutralization: 3-12 μg/mL
  western blot: 0.1-0.2 μg/mL
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   −20°C
Gene Information   human ... IGF1(3479)
biological source   goat
conjugate   unconjugated

Description

Immunogen

recombinant human IGF-I expressed in Escherichia coli

General description

Insulin-like growth factors -1 and -2 are important regulators of signalling that mediates growth and metabolism in cells. Insulin-like growth factor -1 shares structural homology with proinsulin and is mainly produced by liver. IGF-1 acts via specific receptor, IGF-1R, a heterodimeric tyrosine kinase receptor that signals to various second messenger pathways such as MAPK, Raf/Ras and PI3K cascades. The effect of IGF-1 is by endocrine, paracrine and autocrine signalling. The effects of IGF-1 signalling are important at many developmental levels from cells to organ to organism, growth, differentiation, anti-apoptosis, proliferation and transformation. Irregular IGF-1 signaling has been implicated in many cancers including melanoma, breast, lung, colon and prostate
Anti-IGF-I recognizes human insulin-like growth factor I. The antibody does not react with human IGF-II.

Physical form

Lyophilized from a 0.2 μm filtered solution of phosphate buffered saline, pH 7.4, with 5% trehalose.

Specificity

The antibody neutralizes the biological activity of recombinant human IGF-I.

Preparation Note

Affinity isolated by IGF-I affinity chromatography.

Application

Anti- IGF-1 antibody may be used for neutralization reactions, the ND50 of the antibody is 3-12 μg/ml. For immunoblotting a working antibody concentration of 0.1-0.2 μg/ml.

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Safety & Documentation

Documents

Certificate of Analysis

Certificate of Origin

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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