|Related Categories||Antigen-Vaccine Preparation, Biochemicals, Biochemicals and Reagents, Cell Culture, Core Bioreagents,|
|grade||for molecular biology|
|foreign activity||DNase and RNase, none detected|
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May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.
Nonionic, non-denaturing detergent
50, 100 mL in glass bottle
Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.
IGEPAL is a registered trademark of Rhodia Operations
Certificate of Analysis
Certificate of Origin
|Precautionary statements||P273-P280-P305 + P351 + P338|
|Personal Protective Equipment||Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter|
|Hazard Codes (Europe)||Xi,N|
|Risk Statements (Europe)||41-50|
|Safety Statements (Europe)||26-39-61|
|RIDADR||UN 3082 9 / PGIII|
1. Grow cells to ~80% confluency. You will need ~2 million cells/RIP, or ~4 million cells for IgG (neg control) & Ago2 RIP
Keywords: Cell disruption, Immunoprecipitation, Individual protein Immunoprecipitation, RNA immunoprecipitation
Improved quantification of HIV-1-infected CD4+ T cells using an optimised method of intracellular HIV-1 gag p24 antigen detection. Yang H, Yorke E, Hancock G, et al. J. Immunol. Methods 391(1-2), 174-8, (2013)
Quantification of arsenic in dialysate solution and scalp hair samples of kidney failure patients by cloud point extraction and electrothermal atomic absorption spectroscopy. Baig JA, Kazi TG, Arain MB, et al. J. AOAC Int. 95(6), 1755-60, (2012)
Species selective preconcentration and quantification of gold nanoparticles using cloud point extraction and electrothermal atomic absorption spectrometry. Hartmann G and Schuster M Anal. Chim. Acta 761, 27-33, (2013)
Novel coupling of surfactant assisted emulsification dispersive liquid-liquid microextraction with spectrophotometric determination for ultra trace nickel. Deng Q, Chen M, Kong L, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 104, 64-9, (2013)
Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica released by Nonidet P40. Morphew RM, Hamilton CM, Wright HA, et al. Vet. Parasitol. 191(3-4), 379-85, (2013)
Determination of cadmium(II), cobalt(II), nickel(II), lead(II), zinc(II), and copper(II) in water samples using dual-cloud point extraction and inductively coupled plasma emission spectrometry. Zhao L, Zhong S, Fang K, et al. J. Hazard. Mater. 239-240, 206-12, (2012)
Cloud point-dispersive μ-solid phase extraction of hydrophobic organic compounds onto highly hydrophobic core-shell Fe₂O₃@C magnetic nanoparticles. Giokas DL, Zhu Q, Pan Q, et al. J. Chromatogr. A 1251, 33-9, (2012)
Immunoproteomics of Brucella abortus reveals differential antibody profiles between S19-vaccinated and naturally infected cattle. Pajuaba AC, Silva DA, Almeida KC, et al. Proteomics 12(6), 820-31, (2012)
HPTLC method for the simultaneous determination of four indole alkaloids in Rauwolfia tetraphylla: a study of organic/green solvent and continuous/pulse sonication. Gupta S, Shanker K, and Srivastava SK J. Pharm. Biomed. Anal. 66, 33-9, (2012)
LC/MS method using cloud point extraction for the determination of permitted and illegal food colors in liquid, semiliquid, and solid food matrixes: single-laboratory validation. Ates E, Mittendorf K, and Senyuva H J. AOAC Int. 94(6), 1853-62, (2011)
Study on the determination of heavy metals in water samples with ultrasound-assisted dispersive liquid-liquid microextraction prior to FAAS. Li Z, Yu G, Song J, et al. Water Sci. Technol. 67(2), 247-53, (2013)
Simultaneous preconcentration of copper and mercury in water samples by cloud point extraction and their determination by inductively coupled plasma atomic emission spectrometry. Shoaee H, Roshdi M, Khanlarzadeh N, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 98, 70-5, (2012)
Cloud point extraction of copper, lead, cadmium, and iron using 2,6-diamino-4-phenyl-1,3,5-triazine and nonionic surfactant, and their flame atomic absorption spectrometric determination in water and canned food samples. Citak D and Tuzen M J. AOAC Int. 95(4), 1170-5, (2012)
Physiological properties of enkephalin-containing neurons in the spinal dorsal horn visualized by expression of green fluorescent protein in BAC transgenic mice. Fukushima T BMC Neurosci. 12, 36, (2011)
Application of sequential extraction procedures and glycoprotein blotting for the characterization of the 2-D polypeptide patterns of barley seed proteins. Weiss, W., et al. Electrophoresis 13, 770-773, (1992)
A universal method for two-dimensional polyacrylamide gel electrophoresis of membrane proteins using isoelectric focusing on immobilized pH gradients in the first dimension. Schupbach, J., et al. Anal. Biochem. 196, 337-343, (1991)
Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension of barley seed proteins: discrimination of cultivars with different malting grades. Gorg, A., et al. Electrophoresis 13, 192-203, (1992)
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