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I8896 Sigma

IGEPAL® CA-630

for molecular biology

Synonym: Octylphenoxy poly(ethyleneoxy)ethanol, branched

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Caution

May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

Other Notes

Nonionic, non-denaturing detergent

Packaging

1, 50, 100 mL in glass bottle

Quality

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Application

IGEPAL® CA-630 has been used
• as neutral detergent in homogenizing buffer during western blotting of mouse muscle homogenates
• In X-gal staining for studying the lacZ expression in E8.5-E10.5 embryos
• In Cell-Lysis (CL) buffer for preparing RT-qPCR-ready cell lysates

General description

IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.It is chemically indistinguishable from Nonidet P-40.

Legal Information

IGEPAL is a registered trademark of Rhodia Operations

Price and Availability


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Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
2
Flash Point(F) 
483.8 °F
Flash Point(C) 
251 °C

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

1.    Grow cells to ~80% confluency.  You will need ~2 million cells/RIP, or ~4 million cells for IgG (neg control) & Ago2 RIP
Keywords: Cell disruption, Immunoprecipitation, Individual protein Immunoprecipitation, RNA immunoprecipitation

Peer-Reviewed Papers
15

References

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