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I8896 Sigma

IGEPAL® CA-630

for molecular biology

Synonym: Octylphenoxy poly(ethyleneoxy)ethanol, branched

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Description

Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.

Caution

May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

Other Notes

Nonionic, non-denaturing detergent

Packaging

1, 50, 100 mL in glass bottle

Quality

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Application

IGEPAL® CA-630 has been used
• as neutral detergent in homogenizing buffer during western blotting of mouse muscle homogenates1
• In X-gal staining for studying the lacZ expression in E8.5-E10.5 embryos2
• In Cell-Lysis (CL) buffer for preparing RT-qPCR-ready cell lysates3

General description

IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.It is chemically indistinguishable from Nonidet P-40.4

Legal Information

IGEPAL is a registered trademark of Rhodia Operations

Price and Availability

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
2
Flash Point(F) 
483.8 °F
Flash Point(C) 
251 °C

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

1.    Grow cells to ~80% confluency.  You will need ~2 million cells/RIP, or ~4 million cells for IgG (neg control) & Ago2 RIP
Keywords: Cell disruption, Immunoprecipitation, Individual protein Immunoprecipitation, RNA immunoprecipitation

Peer-Reviewed Papers

References

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1. Unmasking potential intracellular roles for dysferlin through improved immunolabeling methods. Roche, J.A., et al. J. Histochem. Cytochem. 59, 964-75, (2011)

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2. Developmental origin of smooth muscle cells in the descending aorta in mice. Johansson, B.R., et al. Development 135, 1823-1832, (2008)

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3. A simple, inexpensive method for preparing cell lysates suitable for downstream reverse transcription quantitative PCR. Shatzkes K, Teferedegne B, and Murata H Sci. Rep. 4, 4659, (2014)

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4. An optimised direct lysis method for gene expression studies on low cell numbers. Viet-Phuong Le A, Huang D, Blick T, et al. Sci. Rep. 5, 12859, (2015)

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Peptides as standard for denaturing isoelectric focusing. Watts, N.R. and Singh, R.P. Electrophoresis 16, 22-27, (1995)

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On the resolution of polypeptides by isoelectric focusing in polyacrylamide gels in the presence of urea and nonidet-p40. Zechel, K. Anal. Biochem. 83, 240, (1977)

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The current state of two-dimensional electrophoresis with immobilized pH gradients. Gorg, A., et al. Electrophoresis 21, 1037-1053, (2000)

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Detergents: an overview. Neugebauer, J. Meth. Enzymol. 182, 239-253, (1990)

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Preparation of membrane fractions. Ozols, J. Meth. Enzymol. 182, 225-235, (1990)

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A universal method for two-dimensional polyacrylamide gel electrophoresis of membrane proteins using isoelectric focusing on immobilized pH gradients in the first dimension. Schupbach, J., et al. Anal. Biochem. 196, 337-343, (1991)

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Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension of barley seed proteins: discrimination of cultivars with different malting grades. Gorg, A., et al. Electrophoresis 13, 192-203, (1992)

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Application of sequential extraction procedures and glycoprotein blotting for the characterization of the 2-D polypeptide patterns of barley seed proteins. Weiss, W., et al. Electrophoresis 13, 770-773, (1992)

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Characterization and purification of an outer membrane metalloproteinase from Pseudomonas aeruginosa with fibrinogenolytic activity. Fricke, B., et al. Biochim. Biophys. Acta 1454, 236, (1999)

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Preparation of extracts from prokaryotes. Cull, M. ,and McHenry, C.S Meth. Enzymol. 182, 147-153, (1990)

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High resolution two-dimensional electrophoresis of proteins. O’ Farrell, P.H. J. Biol. Chem. 250, 4007-4021, (1975)

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Methods for increasing the resolution of two-dimensional protein electrophoresis. Hochstrasser, D.F. Anal. Biochem. 173, 424, (1988)

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Phosphorylation of a membrane curvature-sensing motif switches function of the HOPS subunit Vps41 in membrane tethering. Margarita Cabrera et al J. Cell Biol. 191, 845-59, (2010)

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The chromatin-remodeling factor CHD4 coordinates signaling and repair after DNA damage. Larsen DH J. Cell Biol. 190(5), 731-40, (2010)

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Cloud point sample clean-up and capillary zone electrophoresis with field enhanced sample injection and micelle to solvent stacking for the analysis of herbicides in milk. Kukusamude C, Srijaranai S, Kato M, et al. J. Chromatogr. A 1351, 110-4, (2014)

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Lipoarabinomannan biosynthesis in Corynebacterineae: the interplay of two α(1→2)-mannopyranosyltransferases MptC and MptD in mannan branching. Mishra AK Mol. Microbiol. 80(5), 1241-59, (2011)

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Vortex-assisted surfactant-enhanced emulsification microextraction based on solidification of floating organic drop followed by electrothermal atomic absorption spectrometry for speciation of antimony (ΙΙΙ, V). Eftekhari M, Chamsaz M, Arbab-Zavar MH, et al. Environ. Monit. Assess. 187(1), 4129, (2015)

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Enhancement of anti-cholinesterase activity of Zingiber cassumunar essential oil using a microemulsion technique. Okonogi S and Chaiyana W Drug Discov. Ther. 6(5), 249-55, (2012)

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Methods for chromatographic removal of endotoxin. Lowe AJ, Bardliving CL, and Batt CA Methods Mol. Biol. 899, 265-75, (2012)

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Assessment of gelatinases (MMP-2 and MMP-9) by gelatin zymography. Toth M, Sohail A, and Fridman R Methods Mol. Biol. 878, 121-35, (2012)

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Determination of cadmium(II), cobalt(II), nickel(II), lead(II), zinc(II), and copper(II) in water samples using dual-cloud point extraction and inductively coupled plasma emission spectrometry. Zhao L, Zhong S, Fang K, et al. J. Hazard. Mater. 239-240, 206-12, (2012)

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Species selective preconcentration and quantification of gold nanoparticles using cloud point extraction and electrothermal atomic absorption spectrometry. Hartmann G and Schuster M Anal. Chim. Acta 761, 27-33, (2013)

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DNase 2 is the main DNA-degrading enzyme of the stratum corneum. Fischer H PLoS ONE 6(3), e17581, (2011)

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Cloud point-dispersive μ-solid phase extraction of hydrophobic organic compounds onto highly hydrophobic core-shell Fe₂O₃@C magnetic nanoparticles. Giokas DL, Zhu Q, Pan Q, et al. J. Chromatogr. A 1251, 33-9, (2012)

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Cloud point extraction of copper, lead, cadmium, and iron using 2,6-diamino-4-phenyl-1,3,5-triazine and nonionic surfactant, and their flame atomic absorption spectrometric determination in water and canned food samples. Citak D and Tuzen M J. AOAC Int. 95(4), 1170-5, (2012)

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Cloud point extraction and spectrophotometric determination of mercury species at trace levels in environmental samples. Ulusoy Hİ, Gürkan R, and Ulusoy S Talanta 88, 516-23, (2012)

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Trypanosoma brucei PUF9 regulates mRNAs for proteins involved in replicative processes over the cell cycle. Stuart K. Archer et al PLoS Pathog. 5, e1000565, (2009)

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Katanin knockdown supports a role for microtubule severing in release of basal bodies before mitosis in Chlamydomonas. Rasi MQ Mol. Biol. Cell 20(1), 379-88, (2009)

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Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica released by Nonidet P40. Morphew RM, Hamilton CM, Wright HA, et al. Vet. Parasitol. 191(3-4), 379-85, (2013)

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Simultaneous preconcentration of copper and mercury in water samples by cloud point extraction and their determination by inductively coupled plasma atomic emission spectrometry. Shoaee H, Roshdi M, Khanlarzadeh N, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 98, 70-5, (2012)

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Immunoproteomics of Brucella abortus reveals differential antibody profiles between S19-vaccinated and naturally infected cattle. Pajuaba AC, Silva DA, Almeida KC, et al. Proteomics 12(6), 820-31, (2012)

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Novel coupling of surfactant assisted emulsification dispersive liquid-liquid microextraction with spectrophotometric determination for ultra trace nickel. Deng Q, Chen M, Kong L, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 104, 64-9, (2013)

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A constitutively active form of neurokinin 1 receptor and neurokinin 1 receptor-mediated apoptosis in glioblastomas. Akazawa T J. Neurochem. 109(4), 1079-86, (2009)

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Full factorial analysis of mammalian and avian influenza polymerase subunits suggests a role of an efficient polymerase for virus adaptation. Olive T. W. Li et al PLoS ONE 4, e5658, (2009)

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Effect of the GPI anchor of human Thy-1 on antibody recognition and function. Bradley JE, Chan JM, and Hagood JS Lab. Invest. 93(3), 365-74, (2013)

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Cloud point extraction and flame atomic absorption spectrometry determination of lead (II) in environmental and food samples. Soylak M, Yilmaz E, Ghaedi M, et al. J. AOAC Int. 95(6), 1797-802, (2012)

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HPTLC method for the simultaneous determination of four indole alkaloids in Rauwolfia tetraphylla: a study of organic/green solvent and continuous/pulse sonication. Gupta S, Shanker K, and Srivastava SK J. Pharm. Biomed. Anal. 66, 33-9, (2012)

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The RACK1 signal anchor protein from Trypanosoma brucei associates with eukaryotic elongation factor 1A: a role for translational control in cytokinesis. Regmi S Mol. Microbiol. 70(3), 724-45, (2008)

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Removing endotoxin from plasmid samples by Triton X-114 isothermal extraction. Ma R, Zhao J, Du HC, et al. Anal. Biochem. 424(2), 124-6, (2012)

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Oligomerization of ZFYVE27 (Protrudin) is necessary to promote neurite extension. Pantakani DV, Czyzewska MM, Sikorska A, et al. PLoS ONE 6(12), e29584, (2011)

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Study on the determination of heavy metals in water samples with ultrasound-assisted dispersive liquid-liquid microextraction prior to FAAS. Li Z, Yu G, Song J, et al. Water Sci. Technol. 67(2), 247-53, (2013)

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Improved quantification of HIV-1-infected CD4+ T cells using an optimised method of intracellular HIV-1 gag p24 antigen detection. Yang H, Yorke E, Hancock G, et al. J. Immunol. Methods 391(1-2), 174-8, (2013)

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On a fully closed state of native human type-1 VDAC enriched in Nonidet P40. Thinnes FP and Burckhardt G Mol. Genet. Metab. 107(3), 632-3, (2012)

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LC/MS method using cloud point extraction for the determination of permitted and illegal food colors in liquid, semiliquid, and solid food matrixes: single-laboratory validation. Ates E, Mittendorf K, and Senyuva H J. AOAC Int. 94(6), 1853-62, (2011)

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Quantification of arsenic in dialysate solution and scalp hair samples of kidney failure patients by cloud point extraction and electrothermal atomic absorption spectroscopy. Baig JA, Kazi TG, Arain MB, et al. J. AOAC Int. 95(6), 1755-60, (2012)

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Preparation and characterization of uniform particles of flufenamic acid and its calcium and barium salts. Mohamed AA and Matijević E J. Colloid. Interface Sci. 381(1), 198-201, (2012)

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The interaction between the measles virus nucleoprotein and the Interferon Regulator Factor 3 relies on a specific cellular environment. Colombo M Virol. J. 6, 59, (2009)

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Microvesicle-mediated release of soluble LH/hCG receptor (LHCGR) from transfected cells and placenta explants. Chambers AE Reprod. Biol. Endocrinol. 9, 64, (2011)

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Identification of a terminal rhamnopyranosyltransferase (RptA) involved in Corynebacterium glutamicum cell wall biosynthesis. Birch HL J. Bacteriol. 191(15), 4879-87, (2009)

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Physiological properties of enkephalin-containing neurons in the spinal dorsal horn visualized by expression of green fluorescent protein in BAC transgenic mice. Fukushima T BMC Neurosci. 12, 36, (2011)

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Extraction of the antimicrobial peptide cerein 8A by aqueous two-phase systems and aqueous two-phase micellar systems. Lappe R, Sant'anna V, and Brandelli A Nat. Prod. Res. 26(23), 2259-65, (2012)

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A green method using a micellar system for determination of andrographolide and dehydroandrographolide in human plasma. Zhao Q, Ding J, Jin H, et al. J. Chromatogr. Sci. 51(4), 341-8, (2013)

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