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I9513 Sigma

Monoclonal Anti-Human IgG2 (Fc specific) antibody produced in mouse

clone HP-6002, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Human IgG Secondary Antibodies and Conjugates, Primary Antibodies,
biological source   mouse
antibody form   ascites fluid
clone   HP-6002, monoclonal
contains   15 mM sodium azide
application(s)   indirect ELISA: 1:2,000
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads

Purified human IgG myeloma proteins covalently linked to polyaminostyrene microbeads.

Application

Monoclonal Anti-Human IgG2 (Fc specific) antibody is suitable for use in ELISA.

Monoclonal anti-human IgG2 (Fc specific) antibody can be used to detect IgG2 subclass by using different immunoassays like- hemagglutination (HA), hemagglutination inhibition(HAI), Imprint Immunofixation (IIF) and Particle Counting Immunoassay (PACIA). It can also be used in immunohistological assays.

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders
Monoclonal Anti-Human IgG2 (Fc specific) antibody is specific for an epitope in the Fc region of human IgG2 and does not detect IgG1, IgG3, and IgG4. The association constant of this antibody with its ligand is estimated to be 13x(107) L/M. This product clone has been tested for specificity using various immunoassays in the IUIS/WHO study.

IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane. IgG is further subdivided into four subclass- IgG1, IgG2, IgG3 and IgG4 and contribute 65, 30, 5 and 4% in total IgG. IgG2 produce in response to anti-polysaccharide. Monoclonal anti-human IgG2 (Fc specific) antibody can be used in indirect ELISA and Immunofluorometric Assay (IFMA). Mouse anti-human IgG2 (Fc specific) antibody reacts specifically with IgG2 of human but not with other IgG subclasses.

Price and Availability


Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

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