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I9516 Sigma

2-Propanol

BioReagent, for molecular biology, ≥99.5%

Synonym: sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol

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Properties

Related Categories 2-Propanol (Isopropanol), Core Bioreagents, DNA & RNA Purification, Life Science Reagents for DNA/RNA Electrophoresis, Life Science Reagents for Protein Electrophoresis,
grade   for molecular biology
vapor density   2.1 (vs air)
vapor pressure   33 mmHg ( 20 °C)
  44 mmHg ( 25 °C)
InChI Key   KFZMGEQAYNKOFK-UHFFFAOYSA-N
product line   BioReagent
assay   ≥99.5%
form   liquid
autoignition temp.   750 °F
expl. lim.   2 %
color   colorless
refractive index   n20/D 1.377(lit.)
bp   82 °C(lit.)
mp   −89.5 °C(lit.)
solubility   water: soluble (completely)
density   0.785 g/mL at 25 °C(lit.)
suitability   suitable for precipitation of nucleic acids
storage temp.   room temp

Description

General description

Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly.

Packaging

25, 500 mL in glass bottle

Application

2-Propanol has been used:
• in DNA and RNA extraction
• for determination of hydroxyproline levels
• for checking cell viability by MTT assay
• in microarray hybridization

Price and Availability


Microcon Centrifugal Filters

All labs need water
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN1219 - class 3 - PG 2 - Isopropanol
WGK Germany 
1
RTECS 
NT8050000
Flash Point(F) 
53.6 °F
Flash Point(C) 
12 °C
Protocols & Articles

Articles

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

Isopropanol Molecular Biology Reagent

CAS RN 67-63-0 EC 200-661-7 Synonyms: 2-Propanol, sec-Propyl alcohol, IPA, Isopropyl alcohol
Keywords: Acylations, Biochemistry, Crystallization, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Molecular biology, Precipitation, Size-exclusion chromatography, Solvents

Protocols

MystiCq® MicroRNA® Quantitation System

Technology Overview Workflow Background Protocols miRNA isolation cDNA synthesis miRNA qPCR Advanced information Product selection guides Troubleshooting
Keywords: AGE, Amplification, Buffers, Cell disruption, Centrifugation, Degradations, Diagnostic, Digestions, Diseases, Electrophoresis, Gel electrophoresis, Gene expression, Homogenization, Microarray Analysis, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Sample preparations, Separation, Solvents, Titrations

Protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

1.    Grow cells to ~80% confluency.  You will need ~2 million cells/RIP, or ~4 million cells for IgG (neg control) & Ago2 RIP
Keywords: Cell disruption, Immunoprecipitation, Individual protein Immunoprecipitation, RNA immunoprecipitation

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

TRI Reagent® Protocol

1A. Tissue: Homogenize tissue samples in TRI Reagent (1 ml per 50–100 mg of tissue) in a Polytron® or other appropriate homogenizer.
Keywords: AGE, Buffers, Cell disruption, Centrifugation, Degradations, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Homogenization, PAGE, Polymerase chain reaction, Precipitation, Sample preparations, Separation, Solvents, Western blot

Peer-Reviewed Papers
15

References

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