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IP50 Sigma

Protein G Immunoprecipitation Kit

sufficient for 50 assays




The kit is designed to allow maximal recovery of immunoprecipitates. It provides all the necessary reagents to perform immunoprecipitation from cell extracts of any protein to which a suitable antibody is available. Based on protein G, the kit binds to most commonly used antibodies. In addition, spin columns are provided to enable quick washes without the loss of protein G resin and thus protein yield is maximized.

Features and Benefits

• Minimal loss of antigen-antibody bound beads during washing.
• Minimal or no non-specific signals by increasing the stringency of the washing step.

Preparation Note

When preparing reagents, use ultrapure water (17M -cm)

Price and Availability

Amplified Detection

Kit component also available separately


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5 M Sodium chloride solution 15 mL SDS S6546
Protein G Agarose 2 mL SDS P3296
10% Sodium dodecyl sulfate solution 1 mL SDS 71736
Microcentrifuge tubes (2 ml) 50 ea SDS T2795

Kit component only


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10× IP Buffer 40 mL SDS    
Spin columns and caps 50 ea SDS    
Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
UN 1993 / PGIII
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer - Search 80,000 Antibodies at Sigma-Aldrich

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


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