ISO2 Sigma

Mouse Monoclonal Antibody Isotyping Reagents

sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)

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Properties

Related Categories Antibodies, Antibody Characterization and Analysis, Antibody Isotyping Kits, Antibody Kits, Antibody Purification and Characterization,
usage   sufficient for 1000 tests (clones) (by ELISA)
  sufficient for 40 tests (clones) (by immunodiffusion, ODD)
shipped in   wet ice
storage temp.   2-8°C

Description

Application

Determination of the subclass of a monoclonal antibody is helpful for characterization of the antibody, for choosing detection reagents, and for deciding on a purification scheme.

Kit suitable for use in Ouchterlony Immunodiffusion Assays and ELISA applications.

Features and Benefits

• May be used in a variety of assay formats
• Suitable for all antibody forms
• Determines all mouse IgG subclasses, IgA, and IgM

Specificity

ISO-2 contains 0.2 mL vials of subclass specific antibodies to all four mouse IgG subclasses, IgA, and IgM. Directions are included for using these reagents in ELISA and Ouchterlony assays to determine the subclass of monoclonal antibodies in ascites, culture supernatants, or purified preparations. Additional reagents may be required, such as conjugates for ELISA assays or agarose and buffer components for Ouchterlony assays.

General description

The Mouse Monoclonal Antibody Isotyping Reagents enable qualitative isotype determination of mouse monoclonal antibosies derived from hybridoma supernatant, ascites fluid or purified forms.

Price and Availability

Standard component

Description

Product #

Add to Cart

directions for use with each application    
goat antisera to mouse IgA 0.2 mL    
goat antisera to mouse IgG3 0.2 mL    
goat antisera to mouse IgG1 0.2 mL    
goat antisera to mouse IgG2a 0.2 mL    
goat antisera to mouse IgG2b 0.2 mL    
goat antisera to mouse IgM 0.2 mL    

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