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  • J2253 - Anti-phospho-c-Jun (pSer73) antibody produced in rabbit

J2253 Sigma

Anti-phospho-c-Jun (pSer73) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Gene Regulation, Antibodies for Kinase/Phosphatase Biology,
species reactivity   human, mouse
application(s)   microarray: suitable
  western blot (chemiluminescent): 1:1,000 using extract of mouse NIH3T3 anisomycin or UV-treated cells
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... JUN(3725)
mouse ... Jun(16476)
biological source   rabbit
conjugate   unconjugated



synthetic phosphoserine73 peptide corresponding to residues around Ser73 of human c-Jun, conjugated to KLH.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol


The antibody does not react with non-phosphorylated c-Jun or JunD. It does react with the JunD phosphorylated at Ser100, a site conserved between c-Jun and JunD.

Preparation Note

Purified using protein A and peptide affinity chromatography.


Anti-phospho-c-Jun (pSer73) antibody produced in rabbit has been used in microarray analysis to identify the changes in the level of cellular proteins in cells inoculated with Equine influenza virus.

Biochem/physiol Actions

The protooncogene c-Jun encodes a member of the Jun family that forms a component of the transcription factor AP-1 (Activator Protein-1). It dimerizes with a member of the Fos family via the leucine-zipper motif to form AP-1, a factor involved in binding to TPA (12-O-tetradecanoylphorbol 13-acetate) response element (TRE) of various genes that include human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. The dimers formed are involved in the activation of AP-1 dependent genes. AP-1 regulates the transcription of mammalian DDI (DNA-damage-inducible) genes.

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