• USA Home
  • J4644 - Anti-phospho-JNK1/2 (pThr183/pTyr185) antibody produced in rabbit

EMAIL THIS PAGE TO A FRIEND
J4644 Sigma

Anti-phospho-JNK1/2 (pThr183/pTyr185) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-phospho-SAPK (pTpY183/185), Anti-phospho-Stress Activated Protein Kinase (pTpY183/185), Anti-phospho-c-Jun N-terminal Kinase 1/2 (pTpY183/185)

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins at Phosphothreonine (pThr),
species reactivity   human, rat
application(s)   western blot: 0.2-1.0 μg/mL using human 293 cells treated with UV radiation
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MAPK8(5599)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide derived from a region of JNK1 & 2 that contains threonine183 and tyrosine185.

General description

c-Jun N-terminal kinase (JNK, SAPK) is an extensively studied memebr of the MAPK family of kinases and is critical in mediating the apoptotic signaling. There are three isoforms of JNK, JNK 1, 2 and 3. JNK 1 and 2 are ubiquitously expressed; JNK 3 is restricted to cardiac and neuronal tissues. JNK is phosphorylated by MAPK kinases 4 and 7 at Thr183 and Tyr185, leading to its activation. It is however not clear as to which MAPKK phosphorylates which amino acid residue. The activation of JNK is highly regulated and is in response to the stress signals that the cell faces. JNK phosphorylates c-Jun, the most important member of the AP-1 transcription factor family that is involved in survival, apoptosis, proliferation and tumorigenesis. JNK also activates other transcription factors such as c-myc and p53. JNK is involved in the phosphorylation of proteins such as Bad, Bcl2 and Bim that result in the execution of apoptosis by release of cytochrome c from mitochondria
Anti-phospho-JNK1/2 (SAPK) (pThr183/pTyr185 ) specifically recognizes the endogenous, active forms of JNK1 and 2 phosphorylated at threonine 183 and tyrosine 185.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 50% glycerol, 1% bovine serum albumin (BSA) (IgG and preservatives free) and 0.05% sodium azide.

Application

Anti-phospho-JNK1/2 (pThr183/pTyr185 ) antibody may be used at a working concentration of 0.2-1.0 μg/mL for detection by immunoblotting using human embryonic kidney cells (HEK293T) and HeLa cells.

Price and Availability


Detect Your Unseen Protein

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?