• USA Home
  • J4750 - Monoclonal Anti-JNK, Activated (Diphosphorylated JNK) antibody produced in mouse

J4750 Sigma

Monoclonal Anti-JNK, Activated (Diphosphorylated JNK) antibody produced in mouse

clone JNK-PT48, ~2 mg/mL, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-JNK, Activated (Diphosphorylated JNK)



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, J,
species reactivity   mouse, rat, human
application(s)   immunocytochemistry: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 4-20 μg/mL using extract of human Jurkat cells activated with anisomycin
clone   JNK-PT48, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 46 kDa
  antigen mol wt 54 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MAPK8(5599)
mouse ... Mapk8(26419)
rat ... Mapk8(116554)
biological source   mouse
conjugate   unconjugated



synthetic peptide sequence containing amino acids pTPpYVVTRYYR, corresponding to the phosphorylated form of JNK-activation loop.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


The antibody reacts specifically with the activated diphosphorylated form of JNK. The epitope recognized by the antibody contains the regulatory site of JNK (Thr183 and Tyr185). Weak cross-reaction is observed with the monophosphorylated threonine peptide, but not with the non-phosphorylated or monophosphorylated tyrosine peptides of JNK. It does not recognize any form of p38-MAPK or ERK-MAPK.

Price and Availability

Antibody Explorer

Explore with Confidence
Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?