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J4750 Sigma

Monoclonal Anti-JNK, Activated (Diphosphorylated JNK) antibody produced in mouse

clone JNK-PT48, ~2 mg/mL, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-JNK, Activated (Diphosphorylated JNK)

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, J,
species reactivity   mouse, rat, human
application(s)   immunocytochemistry: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 4-20 μg/mL using extract of human Jurkat cells activated with anisomycin
clone   JNK-PT48, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 46 kDa
  antigen mol wt 54 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MAPK8(5599)
mouse ... Mapk8(26419)
rat ... Mapk8(116554)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide sequence containing amino acids pTPpYVVTRYYR, corresponding to the phosphorylated form of JNK-activation loop.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

The antibody reacts specifically with the activated diphosphorylated form of JNK. The epitope recognized by the antibody contains the regulatory site of JNK (Thr183 and Tyr185). Weak cross-reaction is observed with the monophosphorylated threonine peptide, but not with the non-phosphorylated or monophosphorylated tyrosine peptides of JNK. It does not recognize any form of p38-MAPK or ERK-MAPK.

Price and Availability


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