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K0879 Sigma

Kanamycin sulfate Streptomyces kanamyceticus

powder, γ-irradiated

Synonym: Kanamycin A, Kanamycin sulfate from Streptomyces kanamyceticus, Kanamycin sulfate salt

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Properties

Related Categories Aminoglycosides, Antibiotic powders, Antibiotics, Antibiotics A to Z, Antibiotics G-M,
biological source   from Streptomyces kanamyceticus
grade   for molecular biology
InChI Key   OOYGSFOGFJDDHP-KMCOLRRFSA-N
sterility   γ-irradiated
form   powder
potency   ≥700 μg per mg
color   white to off-white
pH range   6.5 - 8.5 (1% solution)
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
  mycoplasma
storage temp.   2-8°C
suitability   nonselective for Escherichia coli and Coliforms

Description

Application

Suitable for use as a selective agent for bacteria harboring a plasmid conferring kanamycin resistance (recommended use concentration 10-50 μg/mL). Also suitable for prevention of contamination in cell cultures (recommended use concentration 100 μg/mL).

Biochem/physiol Actions

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

Mode of Resistance:Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

Principle

Kanamycin binds to the 30S ribosomal subunit and inhibits protein synthesis, leading to cell death.

Preparation Note

Prepare stock solution directly in the vial with sterile water to a final concentration of 10mg/mL. Stock solution should be stored at 2-8°C.

General description

Kanamycin sulfate is an antimicrobial agent effective against Gram-negative and Gram-positive bacteria. It is used to prevent contamination in cell culture as well as a selective agent for bacteria with kanamycin resistance.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

Price and Availability


Laboratory Gloves

All labs need water
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
NZ3225030
Protocols & Articles
Peer-Reviewed Papers
15

References

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