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  • K1390 - Monoclonal Anti-hKid antibody produced in mouse

K1390 Sigma

Monoclonal Anti-hKid antibody produced in mouse

~1 mg/mL, clone 8C12, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-Kinesin-like 4, KNSL4, Anti-Kinesin-like DNA-binding protein, Anti-OBP ORIP-binding protein, Anti-Origin of plasmid DNA replication-binding protein



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cell and Organelle Proteins, Antibodies to Cytoskeleton,
species reactivity   human
application(s)   immunocytochemistry: suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  western blot: 1-2 μg/mL using HeLa total cell extract
clone   8C12, monoclonal
concentration   ~1 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~65 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... KIF22(3835)
biological source   mouse
conjugate   unconjugated



purified human hKid.

General description

The gene KIF22 (kinesin family member 22) encodes a kinesin-like protein family member that is found to be expressed in human bone, cartilage, joint capsule, ligament, skin, and primary cultured chondrocytes. The gene is mapped to human chromosome 16p11.2.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium axide.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Kinesins hydrolyze ATP to generate energy for molecular motors. The gene KIF22 (kinesin family member 22) encodes a chromokinesin that has the ability to bind to both DNA and microtubules. This mitotic motor protein functions in spindle assembly and proper chromosome segregation during mitosis. It is found to be associated with Chfr (check point protein with FHA (forkhead-associated domain) and RING domains), a ubiquitin-protein isopeptide E3 ligase that is crucial for the control of early mitotic checkpoint.

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Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


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