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K1502 Sigma

α-Ketoglutarate Dehydrogenase from porcine heart

buffered aqueous glycerol solution, 0.1-1.0 units/mg protein (Lowry)

Synonym: Multienzyme 2-oxoglutarate dehydrogenase complex

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Properties

Related Categories 1.2.x.x Acting on aldehydes or oxo groups, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   buffered aqueous glycerol solution
foreign activity   pyruvate dehydrogenase ≤20%
shipped in   dry ice
storage temp.   −20°C

Description

Quality

May contain traces of polyethylene glycol.

Unit Definition

One unit will convert 1.0 μmole of β-NAD to β-NADH per min at pH 7.4 at 30 °C in the presence of saturating levels of coenzyme A.

Physical form

Supplied as a 50% glycerol solution containing ~9 mg per mL bovine serum albumin, 30% sucrose, 1.5 mM EDTA, 1.5 mM EGTA, 1.5 mM 2-mercaptoethanol, 0.3% TRITON X-100, 0.003% sodium azide, and 15 mM potassium phosphate, pH 6.8.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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