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K3393 Sigma

PDK1, active, His tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym: PDPK1, PRO0461

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Properties

Related Categories Active Kinases, Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes,
recombinant   expressed in baculovirus infected Sf9 cells
grade   PRECISIO® Kinase
assay   ≥70% (SDS-PAGE)
form   buffered aqueous glycerol solution
activity   102-138 nmol/min·mg
mol wt   mol wt ~67 kDa
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PDPK1(5170)

Description

Physical form

Supplied in 50mM NaPhosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1mM PMSF, 0.2 mM DTT, 25% glycerol.

Biochem/physiol Actions

PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns (3, 4, 5) P3 or PtdIns (3, 4) P2. PDK1 then activates protein kinase B (PKB) which, in turn, inactivates glycogen synthase kinase-3 (GSK3). The phosphorylation of other proteins by PKB and GSK3 is likely to mediate many of the intracellular actions of insulin. Thus, PDK1 plays a key role in mediating many of the actions of the second messenger(s) PtdIns (3, 4, 5) P3 and/or PtdIns (3, 4) P2. The human PDK1 is a 556-residue monomeric enzyme comprising of a catalytic domain that is most similar to the PKA, PKB and PKC subfamily of protein kinases.

PDPK1 (3-phosphoinositide dependent protein kinase 1) is a serine/threonine protein kinase which is activated upon accumulation of PIP3 (phosphatidylinositol-3,4,5-trisphosphate), a PI3K (phosphoinositide 3-kinase) product. It controls AGC (protein kinase A, G, and C) kinase members, such as AKT (v-akt murine thymoma viral oncogene homolog), p70 ribosomal S6 kinase (S6K), serum- and glucocorticoid-induced protein kinase (SGK), and protein kinase C (PKC) members. It is involved in metabolism, growth, proliferation, and survival. PDPK1 is associated with growth of angiosarcoma cells.

Legal Information

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

Huntington's Disease

Huntington's disease (HD) is an autosomal dominant, late-onset neurodegenerative disorder characterized by a selective neuronal cell death in the cortex and striatum leading to cognitive dysfunction,...
Carolyn L. Crankshaw
BioFiles v7 n2, 2011, 9–14
Keywords: Apoptosis, Huntington Disease, Transcription

Peer-Reviewed Papers
15

References

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