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  • K3502 - Anti-Human Kappa Light Chain (Bound and Free) antibody produced in goat

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K3502 Sigma

Anti-Human Kappa Light Chain (Bound and Free) antibody produced in goat

affinity isolated antibody, lyophilized powder

Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Human Kappa Secondary Antibodies and Conjugates, Primary Antibodies,
application(s)   quantitative precipitin assay: suitable
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   2-8°C
Gene Information   human ... IGK@(50802)
biological source   goat
conjugate   unconjugated

Description

Immunogen

Purified human normal and myeloma κ light chain

General description

Anti-Human κ Light Chain (bound and free) antibody is obtained from goat antiserum. Immunospecific purification removes all goat serum proteins and immunoglobulins that do not bind to the κ light chain (bound and free). The antibody preparation is specific for κ light chains when tested against bound and free light chains. The product shows no reaction with bound or free λ light chains.

Physical form

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2

Application

Anti-Human κ Light Chain (Bound and Free)-FITC antibody was used for as secondary antibody in ELISA at a working antibody dilution of 1:200 using maize seed extracts. For ELISA using NSO/1 myeloma cells, HEK293 cells and mouse plasma, antibody concentration of 1-2 μg/ml was used. The antibody was also used to coat the electrode surface in the preparation of antiatrazine immunosensor.

Reconstitution

Reconstitute with 0.135 M sodium chloride.

Price and Availability


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