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K3753

Krebs-Henseleit Buffer Modified

With 2000 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

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UNSPSC Code:
12352207

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Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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K4002T2397H2387
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

500

Quality Level

500

Quality Level

400

Quality Level

-

form

powder

form

powder

form

liquid

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

General description

Krebs-Henseleit Buffer Modified is a balanced salt solution (BSS) that is used in cell culture and plays a role in providing cells with water and inorganic salts for normal cell metabolism. In addition, it provides a buffering system to maintain the optimal pH of the medium and acts as an irrigating, transporting, and diluting agent to maintain the intra- and extra-cellular osmotic balance. BSS provides glucose as an energy source for cell metabolism.

Application

Krebs-Henseleit Buffer Modified has been used:

  • to collect the rat brains for ex vivo brain slice experiments[1]
  • to measure calcium and sodium in rat pulmonary arterial endothelial cells (PAECs)[2]
  • to collect the uterine and forelimb blood vessel samples from horses[3]

Preparation Note

Formulated to contain 9.6 grams of powder per liter of medium.
Supplement with 2.1 g/L sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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