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  • K4261 - Monoclonal Anti-KSR (Kinase Suppressor of Ras) antibody produced in rat

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K4261 Sigma

Monoclonal Anti-KSR (Kinase Suppressor of Ras) antibody produced in rat

~2 mg/mL, clone C3H7D2, purified immunoglobulin, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, KL-KY,
species reactivity   mouse
application(s)   immunocytochemistry: suitable using methanol-acetone fixation
  microarray: suitable
  western blot: 0.5-1 μg/mL using a whole extract of transfected 293T (human embryonal kidney) cells expressing mouse KSR
clone   C3H7D2, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~100 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Ksr1(16706)
biological source   rat
conjugate   unconjugated

Description

Immunogen

recombinant KSR fragment (amino acids 118-248) from mouse.

General description

Kinase Suppressor of Ras (KSR) is a positive effector of Ras signalling pathway. Initially discovered in Drosophila and C. elegans, the KSR family of proteins is structurally similar but distinct from the Raf Family. In response to Ras activation, KSR translocates from the cytoplasm to the plasma membrane and may act as a scaffolding protein to assemble a MAPK signalling complex. KSR interacts with Raf-1, MEK1/2 and ERK1/2 and this interaction is required for a signalling cascade downstream of Ras. Thus KSR plays an important role in survival, proliferation, differentiation, growth and transformation of cells. KSR mediates Ras-dependent signals that are required for neuronal differentiation and functioning of central nervous system
Monoclonal Anti-KSR specifically reacts with mouse KSR (~100 kDa).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Application

Anti-KSR is suitable for immunoblotting at a working concentration of 0.5-1.0 μg/mL using human 293T cells expressing mouse KSR. The antibody may be used for immunocytochemistry and protein microarray applications.

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