• USA Home
  • K4760 - Anti-Keratinocyte Growth Factor antibody produced in goat

EMAIL THIS PAGE TO A FRIEND
K4760 Sigma

Anti-Keratinocyte Growth Factor antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-FGF-7, Anti-KGF

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Pancreatic Stem Cells, Antibodies for Stem Cell Biology,
species reactivity   human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 15 μg/mL
  indirect ELISA: 0.5-1 μg/mL
  neutralization: suitable
  western blot: 0.1-0.2 μg/mL
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   −20°C
Gene Information   human ... FGF7(2252)
biological source   goat
conjugate   unconjugated

Description

Immunogen

recombinant human keratinocyte growth factor (FGF-7) expressed in E. coli.

General description

Keratinocyte growth factor (KGF, FGF-7) is a member of the fibroblast growth factor (FGF) family and is produced by cells of mesenchymal origin. The receptor for KGF is primarily expressed on the surface of epithelial cells and mediates mesenchymal-epithelial communication. There are multiple functions for KGF including proliferation, migration, DNA repair, differentiation, survival and production of enzymes to nullify the effects of reactive oxygen species. The expression of KGF is reportedly induced by cytokines such as IL-1 and transforming growth factor-α. Some of the pathways that are influenced by KGF are Akt, RSK1 and PAK4. Recent studies implicate KGF in epithelial tumorigenesis under specific situations
Anti-Keratinocyte Growth Factor recognizes human FGF-7. This antibody shows less than 20% cross-reactivity with human FGF-9 and less than 10% cross-reactivity with human FGF-4.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Application

Anti-keratinocyte growth factor antibody may be used to neutralize the biological activity of human KGF at a neutralization dose of ~6-12 μg/ml. The recommended concentration for immunoblotting is 0.1-0.2 μg/ml. For ELISA, a working antibody concentration of 0.5-1.0 μg/ml may be used. Immunohistochemistry of formalin-fixed, paraffin-embedded sections may be performed using a concentration of 15 μg/ml.

Price and Availability


Antibody Explorer

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?